中国生态农业学报2016，Vol.24Issue(4)：506-516,11.DOI:10.13930/j.cnki.cjea.150833
低海拔和高海拔产区气象因子对‘美乐’葡萄浆果品质和代谢组的影响*
Responseof‘Merlot’grapeberryqualityandmetabolometometeorologicalfactorsatbothlowandhighaltitudes
摘要
Abstract
Toillustratetheadaptabilityandqualitycharacteristicsof‘Merlot’grapeberriesinhighaltitudeproductionareas,themetabolitescontentsandmetabolomicsof‘Merlot’grapeberrieswereanalyzedinrelationtometeorologicalfactorsatlow(41m)andhigh(2343m)altitudewine-producingareas.Inthisinvestigation,meteorologicalfactorsatlow/high-altitudeofwine-producing areas were monitored by GPRS-based system. The pH and contents of soluble solids, total acid content,reducingsugars,anthocyanin,totalphenol,tannin,flavonoid,flavoneandproteinsof‘Merlot’grapeberriesweredetermined.Furthermore, GC/TOF-MS technique was used to analyze the difference in metabolome of ‘Merlot’ grape berries betweendifferent altitudes. The results showed that average daily sunshine duration, total radiation during grapes growth period,averagedailyradiation,averagedailytemperaturedifference,averagedailytemperatureandeffectivecumulativetemperatureduringgrowthperiodwerehigherinhigh-altitudethanthoseinlow-altituderegions.Thecontentsofsolublesolid,tanninandreducing sugars of ‘Merlot’ grape berries increased, while the contents of total phenols and anthocyanin decreased inhigh-altitude wine-producing area, compared with those in low-altitude wine-producing area. Metabolic pathway analysisindicated that ‘Merlot’ grape berries in high-altitude wine-producing area accumulated more amino acids, organic acids,alcohols,polyphenolsandsugarsthanthoseinlow-altitudewine-producingarea.Metabolicpathwayenrichmentanalysisshowedthat 8 amino acid metabolome pathways, 4 carbohydrate metabolome pathways, 3 lipid metabolome pathways and 3 nitrogenmetabolomepathwaysof‘Merlot’grapeberrieswereregulatedinhigh-altituderegion.DCCA(DetrendedCanonicalCorrespondenceAnalysis)indicatedthataveragedailysunshineduration, totalradiation, average daily radiation,averagedailytemperaturedifference,averagedailytemperatureandeffectivecumulativetemperatureduringgrowthperiodwerethedrivingfactorsof‘Merlot’ berries quality and metabolites. In conclusion, climatic factors were the main driving factors inducing metabolitedifferencesbetween‘Merlot’grapesberriesgrowinginlow-andhigh-altituderegions.Thus,toadjustmetabolitesprofilesandmetabolicpathwayswasakindofstrategiesof‘Merlot’grapesforadaptingtohighaltitudeecologicalenvironments.关键词
‘美乐’葡萄浆果/海拔梯度/代谢物/代谢组学/气象因子/代谢通路/通路富集Key words
‘Merlot’grapeberry/Altitudegradient/Metabolite/Metabolome/Climaticfactor/Metabolicpathway/Pathwayenrichment分类
农业科技引用本文复制引用
毛如志,张国涛,邵建辉,杜飞,邓维萍,赵新节,朱书生,朱有勇,何霞红..低海拔和高海拔产区气象因子对‘美乐’葡萄浆果品质和代谢组的影响*[J].中国生态农业学报,2016,24(4):506-516,11.基金项目
云南省发改委项目(2014NG005)资助@@@@SupportedbytheprojectofDevelopmentandReformCommissionofYunnanProvince(No.2014NG005) （2014NG005）
