生物安全学报2016，Vol.25Issue(1)：49-53,5.DOI:10.3969/j.issn.2095-1787.2016.01.011
苏云金芽胞杆菌的鉴定及对椰子织蛾的致死作用
ldentification of Bacillus thuringiensis and insect activity against Opisina arenosella Walker
摘要
Abstract
[Background] The biological activity of Bacillus thuringiensis ( Bt) strains collected from soil samples was tested against Opisina arenosella Walker, in order to obtain high insect activity strains that may help control the species. The second objective of this study was to provide new genetic resources for transgenic plant of Bt that could be more resistant to this species. [Method] Soil samples were collected from different areas of Hainan Province potentially suitable for O. arenosella. The temperature screening method was used to isolate Bt strains, which were then identified based on their morphology and molecular biology. The toxicity of Bt strains to larvae of O. arenosella was tested by leaf immersion bioassay method. [Result] Eight Bt isolates were obtained from Baot-ing soil samples, Hainan Province. The 8 Bt isolates included bipyramidal crystal, spherical crystal, square crystal types. PCR-RFLP analysis showed that Cry1, cry1I and cry2 type genes were present in these isolates, but the cry gene-types of two isolates were unknown. The toxicity of the extracted insecticidal crystal proteins (ICP) of the 8 strains against larvae of O. arenosella was as-sayed. The results indicated that the use of ICP of BAT10 ( 50μg��mL-1 ) resulted in 100% mortality of the larvae. ICP of 29-15-4 and BAT20 resulted in more than 80% mortality while the ICP of the other five strains showed less than 50% mortality. [Conclu-sion and significance] This study showed that effective Bt strains could be found in soil potentially suitable to O. arenosella and a-mong them, two strains had the potential to be used as biological control against this species.关键词
Bt/椰子织蛾/鉴定/生物活性Key words
Bacillus thuringiensis/Opisina arenosella Walker/identification/bioactivity引用本文复制引用
孙晓东,阎伟,李朝绪,刘丽,覃伟权..苏云金芽胞杆菌的鉴定及对椰子织蛾的致死作用[J].生物安全学报,2016,25(1):49-53,5.基金项目
海南省重大项目(ZDZX2013008)；海南省重点项目(ZDXM20130049、ZDXM20130004)；海南省基金(311091)；中国热带农业科学院基本科研业务费(1630041014002) （ZDZX2013008）
