生物技术通报2016，Vol.32Issue(4)：48-62,15.DOI:10.13560/j.cnki.biotech.bull.1985.2016.04.006
适配体在食源性致病菌检测中的应用进展
Application Progress of Aptamers in the Detection of Food-borne Pathogenic Bacteria
摘要
Abstract
Aptamers are artificial DNA/RNA oligonucleotides binding target ligands with high affinities,and they are selected from the libraries of single-strand nucleic acid random sequence in vitro by SELEX. Compared with the traditional methods,application of aptamers has the obvious advantages of high specificity,chemical stability and simplicity of modification in the detection of food-borne pathogenic bacteria, thus it draws attentions and applications in this field. However,the combination principle of aptamers to the target has not been understood clearly yet,and summaries on the selection of aptamers against food-borne pathogenic bacteria and its application are still deficient. Combined with the characteristics of food-borne pathogenic bacteria,this article describes the selection characteristics of aptamers against them,and the latest progress in the detection field. Finally,the existing problems and development trend are proposed.关键词
适配体/食源性致病菌/传感器/检测Key words
aptamer/food-borne pathogens/biosensor/detection引用本文复制引用
解沛燕,朱龙佼,许文涛..适配体在食源性致病菌检测中的应用进展[J].生物技术通报,2016,32(4):48-62,15.基金项目
国家高技术研究发展计划(“863”计划,2012AA101606),北京市科技新星计划(XX2014B069) （“863”计划,2012AA101606）
