生物技术通报2016，Vol.32Issue(4)：116-120,5.DOI:10.13560/j.cnki.biotech.bull.1985.2016.04.015
野生大豆根瘤GmGS1γ基因序列分析及原核表达
Sequence Analysis and Prokaryotic Expression of GmGS1γGene from Nodules of Glycine soja
摘要
Abstract
In order to clarify the structures and functions of the members of the glutamine synthetase(GS)gene family,the GmGS1 gene was cloned from Glycine soja by homologous cloning. The bioinformatics analysis showed that,the ORF was 1 071 bp,and the similarity was 100%and 99%with the partial sequence of soybean GS1γ(AF363022.1)and X81700.1,respectively. The sequence had two conserved domains of plant GS,beta-Grasp GS functional area(17-97 aa),and GS catalytic functional area(103-350 aa). Phylogenetic tree showed that GS encoded by the gene GmGS1 probably belonged to glutamine synthetase cytosolic isozyme 2. The gene was expressed in Escherichia coli DE3.0,and the molecular weight of the protein was 44 kD.关键词
野生大豆/谷氨酰胺合成酶(GS)/原核表达/序列分析Key words
Glycine soja/glutamine synthetase(GS)/prokaryotic expression/sequence analysis引用本文复制引用
杨美英,岳胜天,韩红,孙合美,刘晶晶,卢冬雪..野生大豆根瘤GmGS1γ基因序列分析及原核表达[J].生物技术通报,2016,32(4):116-120,5.基金项目
国家自然科学基金项目(31201687) （31201687）
