生物技术通报2016，Vol.32Issue(4)：184-189,6.DOI:10.13560/j.cnki.biotech.bull.1985.2016.04.024
红提葡萄内生细菌的分离鉴定及灰霉病拮抗菌的筛选
Isolation and Identification of Endophytic Bacteria in Red Grape,and Screening of Antagonistic Bacteria Against Botrytis cinerea
摘要
Abstract
This experiment aims to separate the endophytic bacteria,which have the strongly antagonistic effect on Botrytis cinerea, from different parts of red grape in Xinjiang. The tissue isolation was adopted to separate the endophytic bacteria from different parts of red grape,and concurrently 16S rDNA sequence determination and homology analysis were carried out. Meanwhile,the antagonistic effect of endophytic bacteria on B. cinerea was analyzed by confrontation culture method and disc diffusion assay method. The results showed that 5 of 18 strains of endophytic bacteria had solid antagonistic effect,they were NR4-1,NR5-1,NG4-1,NP1-1,and PG1 respectively,and the bacteriostasis rate reached 68.7%.关键词
内生细菌/葡萄灰霉菌/拮抗作用/分离/鉴定Key words
endophytic bacteria/Botrytis cinerea/antagonism/isolation/identification引用本文复制引用
周泠璇,刘娅..红提葡萄内生细菌的分离鉴定及灰霉病拮抗菌的筛选[J].生物技术通报,2016,32(4):184-189,6.基金项目
国家自然科学基金项目(31460031),兵团博士资金专项(2014BB006) （31460031）
