生物技术通报2016，Vol.32Issue(4)：242-250,9.DOI:10.13560/j.cnki.biotech.bull.1985.2016.04.033
微载体浓度与细胞接种密度对ST细胞生长的影响
The Effects of Microcarrier Concentration and Cell Density on the Growth of Swine Testicle Cells
摘要
Abstract
This study is to select the suitable microcarrier concentration and cell seeding density for enhancing the utilization of microcarrier,and optimize the adhered growth and maintenance of ST(swine Testicle)cells. The effects of microcarrier concentration and seeding density on cell growth and maintenance were investigated using 2 different mediums:DMEM supplemented 10%serum or low serum medium(LSM). Further,the utilizations of Cytodex 1 microcarrier by ST cells under different conditions were compared. Results were as below:continuous culture of ST cells in T150 flask using LSM lasted for 30 days,and the average specific growth rate was 0.626 d-1,which was 1.15 times of that using DMEM supplemented with 10%FBS. The maximum cell density was 38.3×10 cells/mL and the utilization was up to 58.8%when microcarrier concentration was 3 g/L Cytodex 1 in mixing bottle and seeding density was 10×105 cells/mL. Furthermore,when cultured in perfusion system for 15 days,the final ST cell density reached 36.6×105 cells/mL,which amplified 14.6 times. Conclusively,the uses of serum and microcarrier are favorable for ST cell adhesion and growth to achieve high cell density,but also this increases the cost of introducing microcarrier. Maximum utilization of microcarrier and nutrients in the medium,and the optimal growth and maintenance could be achieved through selecting suitable microcarrier concentration and seeding density. The results in this work provide guidance for further industrial-scale microcarrier culture system producing CSFV vaccine.关键词
微载体/ST细胞/猪瘟病毒/细胞密度Key words
microcarrier/ST cells/classical swine fever virus/cell density引用本文复制引用
陈以衡,陶姝宇,刘旭平,谭文松..微载体浓度与细胞接种密度对ST细胞生长的影响[J].生物技术通报,2016,32(4):242-250,9.基金项目
国家自然科学基金项目(21206040,21406066),国家“863”计划项目(2012AA02A303),国家重大专项(2013ZX10004003-003-003),中央高校基本科研业务费(WF1214035) （21206040,21406066）
