山西农业大学学报(自然科学版)2016,Vol.36Issue(4):257-261,5.
芥菜型油菜中 SK2型脱水蛋白的表达对大肠杆菌抗逆性的影响
Expression of Brassica j uncea SK2-type dehydrin gene enhances stress tolerance in Escherichia coli
孙雅慧 1罗桂花 1柴团耀 2王慧春 1徐进1
作者信息
- 1. 青海师范大学 生命与地理科学学院,青海 西宁 810008
- 2. 中国科学院大学 生命科学学院,中国 北京 100049
- 折叠
摘要
Abstract
To elucidate the contribution of BjDHN2/3 to abiotic stress tolerance ,the prokaryotic expression vector pET30a‐BjDHN2/3 was constructed and then transferred into E .coli strain BL21.Compared with control strain con‐tainingtheemptyvectorwithoutBjDHN2/3gene,E.coliexpressingtheBjDHN2/3showimprovedtolerancewhen exposed to cold ,drought and NaCl .The results indicated the overexpressing BjDHN2/3 directly contributed to en‐hancing tolerance of transgenic E .coli under stress conditions .关键词
芥菜型油菜/脱水蛋白/大肠杆菌/胁迫耐受性Key words
Brassica juncea/Dehydrin/Escherichia coli/Stress tolerance分类
生物科学引用本文复制引用
孙雅慧,罗桂花,柴团耀,王慧春,徐进..芥菜型油菜中 SK2型脱水蛋白的表达对大肠杆菌抗逆性的影响[J].山西农业大学学报(自然科学版),2016,36(4):257-261,5.