实用医学杂志2016，Vol.32Issue(6)：887-890,4.DOI:10.3969/j.issn.1006-5725.2016.06.006
5-氮杂-2'-脱氧胞苷联合曲古霉素A对高糖诱导下胰岛β细胞的增殖及PDX-1甲基化的影响
Effects of 5-aza-2' -deoxycytidine combined with trichostatin A on cell proliferation and PDX-1 methylation on high glucose-induced toxicity in pancreatic β cells
摘要
Abstract
Objective To investigate the effects of 5-aza-2'-deoxycytidine (5-Aza-dC) alone or combined with trichostatin A(TSA) on cell proliferation, promoter methylation and mRNA expression level of PDX-1 gene in pancreatic β cells induced by high glucose toxicity. Method NIT-1 cells were treated in vitro by high glucose (33.3 mmol/L), then divided into five groups, control group, HG grpup, 5-Aza-dC treatment group, TSA interfere group and 5-Aza-dC + TSA group. Proliferation of NIT-1 cells, insulin secretion, promoter methylation and mRNA expression of PDX-1 gene were detected respectively. Results 5-Aza-dC and TSA alone or in combination could promote cell proliferation and recover insulin secretion in NIT-1 cells , could also reduce PDX-1 gene methylation and enhance expression of PDX-1 mRNA. Compared with single-treatment group , combined group was significantly different (all P < 0.05). Conclusion 5-Aza-dC and TSA could activate the expression of PDX-1 and, then recover insulin secretion in NIT-1 cells induced by high glucose. Combination of them had synergistic effect.关键词
高浓度葡萄糖/DNA甲基化/5-氮杂-2′-脱氧胞苷/曲古霉素AKey words
High glucose/DNA methylation/5-aza-2'-deoxycytidine(5-Aza-dC)/Trichostatin A(TSA)引用本文复制引用
张文静,王利,陈莉,郭丽银,赵娟,邵菁,王红祥..5-氮杂-2'-脱氧胞苷联合曲古霉素A对高糖诱导下胰岛β细胞的增殖及PDX-1甲基化的影响[J].实用医学杂志,2016,32(6):887-890,4.基金项目
武汉市卫生局课题(编号:WX12C22) （编号:WX12C22）
