实用医学杂志2016，Vol.32Issue(6)：890-893,4.DOI:10.3969/j.issn.1006-5725.2016.06.007
吉非替尼衍生物对体外间变形星形细胞瘤增殖的影响
Effecte of the derivative of Gefitinib on proliferation of astrocytoma in vitro
摘要
Abstract
Objective The derivative of Gefitinib was used to treat glioma cells in vitro to explore a more effective new drug for the clinical treatment of astrocytoma. Methods (1) Fifteen kinds of gefitinib derivatives, gefitinib and temozolomide were used to treat glioma cells, and the effect of 0, 10, 15, 20, 25 and 30 μmol/L of each kind of drug on cell proliferation was detected by by MTT assay , respectively. (2) To calculate the concentration of IC50 , then select lower IC50 of derivativs combinate gefitinib and temozolomide with 10, 20 and 30 μmol/L to treat cells, then the apoptosis of cells were detected by flow cytometry. Expression of p-EGFR was detected by western–blot assay. Results (1) NO.LPY-5,9,11, but not other derivatives of Gefitinib could effectively inhibit the growth of cells. (2) IC50 of NO.LPY-9 was less than that of the 5th drug, and both of them were lower than those of gefitinib and temozolomide; NO. LPY-11 was excluded. (3) The cell apoptosis of No. LPY-9 was higher than that of gefitinib and temozolomide , respectively. However, No.LPY-9-induced cell apoptosis was significantly higher than that of No. LPY-5-induced cell. (4) Levels of p-EGFR expression in No.LPY-9 and gefitini-induced cells were significantly lower than that in the negative control group. Conclusion No.LPY-9 has asignificant inhibitory effect on glioma cells in vitro , resulting from the inhibition of the ERFR-mediated signaling pathways and induction of cell apoptosis.关键词
人胶质瘤细胞/吉非替尼/吉非替尼衍生物/细胞凋亡Key words
Human glioma cells/Gefitinib/The ramification of gefitinib/Cell Apoptosis引用本文复制引用
刘来兵,出良钊,刘健,杨华,李玉美..吉非替尼衍生物对体外间变形星形细胞瘤增殖的影响[J].实用医学杂志,2016,32(6):890-893,4.基金项目
国家自然科学基金项目(编号:81560409) （编号:81560409）
教育部"创新团队发展计划"项目[编号:国家教育部科技司(教技函509-IRT13508)] （教技函509-IRT13508）
贵阳市社会发展与民生科技计划项目(编号:筑科合同[2012]103.39号) （编号:筑科合同[2012]103.39号）
贵州省科技局联合基金项目(编号:黔科合LG字[2012]069号) （编号:黔科合LG字[2012]069号）
