特产研究2016，Vol.38Issue(1)：13-20,8.DOI:10.16720/j.cnki.tcyj.2016.01.004
人参细胞TTC－脱氢酶活力测定条件的优化
The Optimization of TTC -dehydrogenase Method for Determination of Panax ginseng Cell Activity
摘要
Abstract
This article focused on the optimization of test conditions of Panax ginseng cells activity through setting L16 (45 ) orthogonal test and single factor test by using 2 ,3 ,5 -TriphenyltetrazolimChloride dehydrogenase assay .Taking TTC concentration ,pH ,reaction temperature and time as four factors of test ,this paper explore the best test condition of Panax ginseng cells activity by using TTC dehydrogenase assay based on OD and U value as reviewindicators .The results showed reaction temperature and time had significantly influence on ginseng cells activity (P<0.1) ,especially the reaction temperature(P<0.01) ,We conclude that the optimum condition in determination of gensing cells vitality as:0.4% TTC ,pH 7.5 ,reaction temperature 25℃ ,reaction time 18h .关键词
人参愈伤组织/2,3,5-氯化三苯四氮唑-脱氢酶还原法/单因子试验设计/正交设计/细胞活力Key words
Panax ginseng callus/TTC-dehydrogenase method/single factor test/orthogonal test/cell activity分类
农业科技引用本文复制引用
王晗,雷秀娟,宋娟,尹红新,姚丽娜,梁韶,侯志芳,王英平..人参细胞TTC－脱氢酶活力测定条件的优化[J].特产研究,2016,38(1):13-20,8.基金项目
吉林省青年科研基金项目 （）
