特产研究2016，Vol.38Issue(1)：39-43,5.DOI:10.16720/j.cnki.tcyj.2016.01.010
HPLC－ESI－MSn分析不同品种山葡萄中花色苷成分
Analysis of Anthocyanins in Different Vitis amurensis Rupr . Species by HPLC -ESI-MSn
摘要
Abstract
The aim of the present study is to analyze five anthocyanins in Vitis amurensis Rupr .by HPLC-ESI-MSn .And the contents of five anthocyanins in five different V .amurensis Rupr .species were compared .Column:PFP Kinetix (2.1mm × 50mm ,2.6μm );mobile phase:2%formic acid water and 2% formic acid acetonitrile under gradient elution .The ESI-MS was carried out in negative ion mode and multiple reac-tion monitoring (MRM ) .The results demonstrated that the method developed was accurate and rapid with good reproducibility and high resolu-tion .This method could be applied in the quantitative analysis of anthocyanins in V .amurensis Rupr .and related products .关键词
高效液相色谱-质谱联用法/花色苷单糖苷/山葡萄/定量分析Key words
HPLC-ESI-MSn/anthocyanin/Vitis amurensis Rupr ./quantitative analysis分类
生物科学引用本文复制引用
刘炎,黄鑫,杨义明,艾军..HPLC－ESI－MSn分析不同品种山葡萄中花色苷成分[J].特产研究,2016,38(1):39-43,5.基金项目
国家自然科学基金青年基金项目（31500269）；中国农业科学院中央级公益性科研院所基本科研业务费专项 （）
