陕西科技大学学报（自然科学版）2016，Vol.34Issue(2)：6-11,6.
漆酶的形态特性表征及固定化研究
Study on morphological characterization and immobilization of laccase
摘要
Abstract
The use of immobilized laccase in papermaking wastewater treatment can signifi-cantly reduce the residual lignin content in water .The use of SEM ,XRD ,laser particle size , energy spectrum analysis can provide an effective evaluation method for laccase immobiliza-tion .The molecular size of the laccase was mainly concentrated in the 11 .482~19 .953 μm . The principle for immobilization of laccase by sodium alginate and PVA were internal embed-ding and surface adsorption respectively .The particle size and the number of the enzyme molecules in the sodium alginate embedding method were large and fixed firmly and not easy to fall off ;Epoxidized PVA immobilized laccase tended to absorb small particles and vulnera-ble to environmental impact ,so the fixed affect was poor .Under the same activity ,the crys-tallinity of the immobilized laccase by sodium alginate was higher than that of the PVA ad-sorption method .The qualitative analysis of the sulfur and sodium elements confirmed the effect of laccase immobilization .关键词
木质素/固定化漆酶/SEM/XRD/形态分析Key words
lignin/immobilized laccase/SEM/XRD/morphological analysis分类
资源环境引用本文复制引用
张安龙,郗文君,杜飞,周丹妮,杨楠..漆酶的形态特性表征及固定化研究[J].陕西科技大学学报（自然科学版）,2016,34(2):6-11,6.基金项目
国家十二五科技支撑计划项目（2011BAC11B04）；陕西科技大学博士科研启动基金项目 （）
