现代妇产科进展2016，Vol.25Issue(4)：241-244,4.DOI:10.13283/j.cnki.xdfckjz.2016.04.001
siRNA沉默KLF4表达促进HeLa细胞上皮间质化
Silencing the expression of KLF4 by siRNA improve the epithelial mesenchymal transi-tion of Hela cells
摘要
Abstract
Objective:To investigate the effect of Klf4 on the EMT of Hela. Method:siRNA targeted to Klf4 and control siRNA was designed and transfected into Hela cells. DMEM with 10ng/ml TGF-β1 and 10% fetal calf serum(FBS)were used to induce the epithelial mes-enchymal transition ( EMT) of Hela. Culture medium without TGF-β1 was taken as control. The morphology of Hela cells were observed and recorded by phrase contrast microscope. The ex-pressions of Klf4,E-cadherin,ZO-1 and N-cadherin were detected by Western blot and Immu-nofluorescence. Results:Hela cells acquired mesenchymal features, showed spindle-shape, fi-broblast-like morphology and EMT aroused under the induction of TGFβ1 . The expressions of Klf4,epithelial marker E-cadherin, ZO-1 were down-regulated while mesenchymal marker N-cadherin was up-regulated in Hela cells induced by TGFβ1 both in Western blot and Immuno-fluorescence. Compared with cells transfected by control siRNA, the expression of epithelial marker E-cadherin,ZO-1 were down-regulated slightly while the mesenchymal marker N-cad-herin was up-regulated significantly after Hela cells were transfected by siKLF4 and induced by TGFβ1 both in Western blot and Immunofluorescence. Conclusion:The EMT of Hela is im-proved after inhibiting the expression of Klf4 by siRNA.关键词
Kruppel样因子4/上皮间质转化/hela细胞/转化生长因子β1Key words
Klf4/EMT/Hela cells/TGF-β1分类
医药卫生引用本文复制引用
任晓丽,宋晖,刘海霞,魏莉,陈必良..siRNA沉默KLF4表达促进HeLa细胞上皮间质化[J].现代妇产科进展,2016,25(4):241-244,4.基金项目
国家自然科学基金(No:81301921) （No:81301921）
陕西省自然科学基金(No:2013JM4023) （No:2013JM4023）
