陈丽 1李彩霞 2郭静 1罗宁 2杨伟红 1程忠平2
作者信息
- 1. 同济大学附属杨浦医院妇产科，上海 200090
- 2. 同济大学医学院妇科微创医学研究所，上海 200090
- 折叠
摘要
Abstract
Objective:To demonstrate the discrepancy of hypoxia-induced apoptosis in eutopic endometrium with adenomyosis( EuE) and ectopic endometrium with adenomyosis( EE) after laparoscopic bilateral laparoscopic uterine artery occlusion ( LUAO) therapy. Methods:The tissues of EuE and EE were collected 30 min after LUAO during surgery and evaluated,be-fore LUAO tissus were collected as control. Then,Real-time PCR and Western blot were per-formed to analyze the mRNA and protein levels of hypoxia-induced apoptosis-related molecules including Bcl-2, Bax, cyt-c, AIF, caspase-3, caspase-4, caspase-8, caspase-9, Endo-G, apf-1, GRP78,CHOP and TRADD. Results:We found that endogenous mitochondrial apoptosis path-way core factors the Bcl-2,Bax,cyt-c,AIF,caspase-3,caspase-9,Endo - G,apf -1 expression increased significantly. Conclusion:Endogenous mitochondrial apoptosis pathway mainly ac-counts for the effects of LUAO therapy on adenomyosis,accompanied by endoplasmic reticulum stress,which results cell apoptosis at last. It was relative with the good therapeutic effect of ade-nomyosis after laparoscopic uterine occlusion.关键词
子宫腺肌病/在位子宫内膜/病灶子宫内膜/子宫动脉阻断/凋亡Key words
Adenomyosis/Eutopic endometrium/Ectopic endometrium/Uterine artery occlusion/Apoptosis分类
医药卫生
