中国组织工程研究2016，Vol.20Issue(1)：83-88,6.DOI:10.3969/j.issn.2095-4344.2016.01.015
内皮样细胞与脐静脉内皮细胞的比较
Endothelial-like cellsversus human umbilical vein endothelial cells
郝晓娟 1郝海英 1朱敏杰 1袁铮 1李伟伟 1陈洁 1朱旅云2
作者信息
- 1. 邯郸市中心医院肾内二科，河北省邯郸市 056001
- 2. 白求恩国际和平医院，河北省石家庄市 050000
- 折叠
摘要
Abstract
BACKGROUND:Stem cels are induced to differentiate into endothelial-like cels that can be used for the treatment of diabetic lower extremity vascular disease. However, it is unclear whether these endothelial-like cels can completely replace endothelial cels to improve vascular disease and what are the differences between endothelial-like cels and endothelial cels. OBJECTIVE:To explore the differences and similarities between endothelial-like cels and human umbilical vein endothelial cels in the aspects of morphology, function, and viability. METHODS:Umbilical cord mesenchymal stem cels and umbilical vein endothelial cels were isolated, cultured and identified using flow cytometry and immunohistochemical method. Isolated umbilical cord mesenchymal stem cels were induced in DMEM-LG/F12 containing 10 µg/L vascular endothelial growth factor, 10 µg/L basic <br> fibroblast growth factor and 2% fetal bovine serum to differentiate into endothelial-like cels folowed by immunohistochemical identification. To compare endothelial-like cels with human umbilical vein endothelial cels, cel migration detection, active substance measurement and three-dimensional angiogenesis test were performed. RESULTS AND CONCLUSION:Isolated umbilical cord mesenchymal stem cels strongly expressed the surface markers of mesenchymal stem cels, and human umbilical vein endothelial cels strongly expressed CD31 and VWF. After induction, the umbilical cord mesenchymal stem cels were identified to highly express CD31 and VWF. Through cel migration, active substance and three-dimensional angiogenesis tests, endothelial-like cels were similar to endothelial cels in the function and activity, and superior to endothelial cels. Cite this article:Hao XJ, Hao HY, Zhu MJ, Yuan Z, Li WW, Chen J, Zhu LY. Endothelial-like cels versus human umbilical vein endothelial cels. Zhongguo Zuzhi Gongcheng Yanjiu. 2016;20(1):83-88.关键词
干细胞/培养/脐带间充质干细胞/内皮样细胞/内皮细胞/细胞迁移/血管形成Key words
Umbilical Cord/Mesenchymal Stem Cels/Endothelial Cels/Cel Migration Assays/Tissue Engineering分类
医药卫生引用本文复制引用
郝晓娟,郝海英,朱敏杰,袁铮,李伟伟,陈洁,朱旅云..内皮样细胞与脐静脉内皮细胞的比较[J].中国组织工程研究,2016,20(1):83-88,6.
