中国组织工程研究2016，Vol.20Issue(1)：118-122,5.DOI:10.3969/j.issn.2095-4344.2016.01.021
异氟醚对新生大鼠海马神经干细胞增殖及分化的影响
Isoflurane effects on the proliferation and differentiation of neural stem cells in the hippocampus of neonatal rats
闵娜 1胡强夫 1李晓培 1聂晓红 1杨利利1
作者信息
- 1. 郑州大学第五附属医院麻醉科，河南省郑州市 450052
- 折叠
摘要
Abstract
BACKGROUND:Isoflurane is an anesthesia drug that has a certain effect on the nervous system. It possibly causes neurologic disorders through impacting nerve stem cel function or morphology. OBJECTIVE:To investigate the effects of isoflurane on the proliferation and differentiation of neural stem cels in the hippocampus of rats. METHODS:Neural stem cels from the hippocampus of neonatal Sprague-Dawley rats, aged 7 days, were induced and differentiated. Passage 3 cels were obtained and divided into two groups: isoflurane group (a mixture gas of 2.8% isoflurane, 5% CO2 and 95% O2) and control group (a mixture of 5% CO2 and 95% O2). <br> After intervention of 6 hours folowed by 2 hours of routine culture, anti-BrdU monoclonal antibody immunofluorescent staining was used to detect cel proliferation, and western blot assay to detect the expression of β3-tubulin and glial fibrilary acidic protein. RESULTS AND CONCLUSION:Compared with the control group, the number of BrdU positive cels in the isoflurane group reduced significantly, indicating that isoflurane inhibits the proliferation of neural stem cels. Compared with the control group, the expression of glial fibrilary acidic protein in the isoflurane group up-regulated, but the expression of β3-tubulin had no changes, indicating isoflurane promotes the differentiation of neural stem cels into astrocytes. Cite this article:Min N, Hu QF, Li XP, Nie XH, Yang LL.Isoflurane effects on the proliferation and differentiation of neural stem cels in the hippocampus of neonatal rats. Zhongguo Zuzhi Gongcheng Yanjiu. 2016;20(1):118-122.关键词
干细胞/培养/异氟醚/吸入麻醉/大鼠/新生/海马/神经干细胞/细胞增殖/神经元分化Key words
Neural Stem Cels/Isoflurane/Anesthesia, Inhalation/Cel Proliferation/Tissue Engineering分类
医药卫生引用本文复制引用
闵娜,胡强夫,李晓培,聂晓红,杨利利..异氟醚对新生大鼠海马神经干细胞增殖及分化的影响[J].中国组织工程研究,2016,20(1):118-122,5.
