中国组织工程研究2016，Vol.20Issue(5)：622-627,6.DOI:10.3969/j.issn.2095-4344.2016.05.003
丹参接骨胶囊对闭合性股骨骨折模型大鼠骨折愈合的影响
Effects of salvia miltiorrhiza bone-setting capsule on fracture healing in a rat model of closed femoral fractures
摘要
Abstract
BACKGROUND:Salvia miltiorrhiza bone-setting capsule is a traditional Chinese medicine for the treatment of fractures due to activating blood circulation to dissipate blood stasis, reducing sweling and pain. OBJECTIVE: To observe the effects of Salvia miltiorrhiza bone-setting capsule on the fracture healing in a rat model of closed femoral fractures. METHODS: Rats were randomly divided into salvia miltiorrhiza bone-setting capsule group, physiological saline group and normal group. In the salvia miltiorrhiza bone-setting capsule group and physiological saline group, rat models of closed femoral fractures were prepared, and then given physiological saline and salvia miltiorrhiza bone-setting capsule 2 pils by intragastric administration. In the normal group, rats were housed normaly. At 7, 14 and 28 days after fractures, hematoxylin-eosin staining conditions, serum osteocalcin, the expression of colagen type I, and the expression of protein and mRNA calus transforming growth factor-beta 1 were observed in the salvia miltiorrhiza bone-setting capsule group and physiological saline group. RESULTS AND CONCLUSION: (1) Hematoxylin-eosin staining demonstrated that at 7 days after fractures, no significant difference was found in pathological changes of femoral fracture in salvia miltiorrhiza bone-setting capsule group and physiological saline group. At 14 and 28 days after fractures, pathological repair was more obvious in the salvia miltiorrhiza bone-setting capsule group than in the physiological saline group. (2) At 3 and 7 days after fractures, serum osteocalcin and the expression of type I colagen were significantly increased in the salvia miltiorrhiza bone-setting capsule group and physiological saline group (P < 0.05), and the expression trend was consistent in both groups. The expression was always higher in the salvia miltiorrhiza bone-setting capsule group than in the physiological saline group, and significant differences were found at 14 and 28 days after fractures (P < 0.01). (3) Transforming growth factor beta 1 expression reached a peak at 3 days after fractures, gradualy reduced, increased at 14 days (the second peak), and diminished at 28 days in the salvia miltiorrhiza bone-setting capsule group and physiological saline group. The expression trend of transforming growth factor beta 1 was consistent in the salvia miltiorrhiza bone-setting capsule group and physiological saline group. At 7, 14 and 28 days, the transforming growth factor beta 1 expression was higher in the salvia miltiorrhiza bone-setting capsule group than in the physiological saline group. (4) Results showed that salvia miltiorrhiza bone-setting capsule could promote fracture healing, and its mechanism was probably associated with serum osteocalcin, the expression of colagen type I and transforming growth factor-β1.关键词
实验动物/骨软骨损伤与修复动物模型/丹参接骨胶囊/骨折愈合/骨钙素/Ⅰ型胶原/转化生长因子β1/苏木精-伊红染色/SD大鼠/闭合性股骨骨折/ELISA/RT-PCR/辽宁省自然科学基金分类
医药卫生引用本文复制引用
王希强,孙仁光,孙仕润,孙军,王咏梅,刘太建,杜辉君,潘峰..丹参接骨胶囊对闭合性股骨骨折模型大鼠骨折愈合的影响[J].中国组织工程研究,2016,20(5):622-627,6.基金项目
辽宁省自然科学基金(2013021023)@@@@Tissue Engineering （2013021023）
Fractures, Bone （）
Healing （）
Femur （）
Models, Animal @@@@ the Natural Science Foundation of Liaoning Province, No.2013021023 （）
