中国组织工程研究2016，Vol.20Issue(5)：646-651,6.DOI:10.3969/j.issn.2095-4344.2016.05.007
糖痹康对糖尿病模型大鼠坐骨神经胰岛素样生长因子Ⅰ及其受体表达的影响
Effects ofTangbikang on insulin like growth factor-I and its receptor expression in sciatic nerves of diabetic rats
摘要
Abstract
BACKGROUND:Chinese medicineTangbikang can improve nerve conduction velocity in diabetic rats, and has anti-inflammatory and antioxidant effects. Insulin like growth factor-I is a key target in the treatment of diabetic peripheral neuropathy. OBJECTIVE:To observe the effect ofTangbikangon the expression of insulin-like growth factor-I and its receptor protein and mRNA in the sciatic nerve of diabetic rat model. METHODS:The experimental diabetes melitus rat models were induced by feeding high fat forage and injection with streptozotocin. After model establishment, rats were givenTangbikang 4.18, 8.35, 16.7 mg/kg per day. This study set positive control methycobal, model and normal control groups. Intragastric administration was performed for 16 weeks. RESULTS AND CONCLUSION: Compared with the model group, blood glucose levels were similar in the methycobal group, but decreased in the high-doseTangbikang group (P < 0.01). Immunohistochemical staining and real-time fluorescence quantitative PCR detection revealed that body mass, motor nerve conduction velocity, insulin like growth factor-I and its receptor protein and mRNA expressions were increased in the methycobal and high-dose Tangbikang groups (P< 0.05 orP < 0.01). Results indicated that Tangbikang can prevent and treat diabetic peripheral neuropathy by promoting insulin like growth factor-I and its receptor. Cite this article:Mu XH, Sun W, Qin LL, Wu LL, Li WL, Guo X, Zhang L, Liu TH.Effects of Tangbikang on insulin like growth factor-I and its receptor expression in sciatic nerves of diabetic rats. Zhongguo Zuzhi关键词
实验动物/神经损伤与修复动物模型/糖痹康/大鼠/糖尿病周围神经病变/胰岛素样生长因子Ⅰ/胰岛素样生长因子Ⅰ受体/免疫组织化学/实时荧光定量PCR/蛋白表达/基因表达/国家自然科学基金分类
医药卫生引用本文复制引用
穆晓红,孙文,秦灵灵,吴丽丽,李伟笠,郭璇,张露,刘铜华..糖痹康对糖尿病模型大鼠坐骨神经胰岛素样生长因子Ⅰ及其受体表达的影响[J].中国组织工程研究,2016,20(5):646-651,6.基金项目
国家自然科学基金(81202970)@@@@Tissue Engineering （81202970）
Diabetes Melitus （）
Sciatic Nerve @@@@ the National Natural Science Foundation of China, No.81202970 （）
