中国组织工程研究2016，Vol.20Issue(5)：694-700,7.DOI:10.3969/j.issn.2095-4344.2016.05.015
活性维生素D3干预保护2型糖尿病模型大鼠肝脏的作用机制
Action mechanisms of active vitamin D3 on protecting the liver of type 2 diabetes mellitus rats
摘要
Abstract
BACKGROUND:Active vitamin D3 plays an important role in the development of type 2 diabetes and its complications. OBJECTIVE: To explore the influence of active vitamin D3 on the liver of type 2 diabetes melitus rats, and its mechanisms. METHODS:A total of 35 male Sprague-Dawley rats were randomly divided into normal control group, diabetes group and vitamin D3 group. In the diabetes group and vitamin D3 group, rats received high fat and high sugar diet and intraperitoneal injection of streptozotocin to prepare rat models of diabetes melitus. In the vitamin D3 group, rats were intragastricaly given calcitriol dissolved in peanut oil 0.03 μg/kg per day. In the normal control group and diabetes group, rats were intragastricaly given peanut oil. 8 weeks later, rats were sacrificed and serum was isolated. Fasting blood glucose, fasting insulin, total cholesterol and triacylglycerol levels were measured. Insulin resistance index in the steady-state model was calculated. The liver was retained, and subjected to hematoxylin-eosin staining, immunohistochemical staining, and real-time fluorescent quantitative PCR. RESULTS AND CONCLUSION: (1) Compared with the diabetes group, triacylglycerol and insulin resistance index were lower in the vitamin D3 group (P < 0.05). (2) Compared with the normal control group, sweling of the liver cels and fatty degeneration with inflammatory cel infiltration were found. Protein expression of JNK and C-Jun and phosphorylation levels, mRNA expression of JNK and C-Jun, tumor necrosis factor α and interleukin-1β increased in the diabetes group (P < 0.05). Liver cel sweling and fatty degeneration lessened, inflammatory cel infiltration reduced in the vitamin D3 group. Simultaneously, the expression of above factors was lower in the vitamin D3 group than in the diabetes group (P < 0.05). (3) Results suggested that the protective effect of vitamin D3 on the liver of rats with type 2 diabetes was possibly associated with its effect on downregulating JNK/C-Jun signaling pathway.关键词
实验动物/内分泌系统损伤与修复动物模型/活性维生素D3/2型糖尿病/SD大鼠/肝脏/C-Jun氨基末端激酶/炎症/胰岛素抵抗/糖尿病动物模型/链脲佐菌素/国家自然科学基金分类
医药卫生引用本文复制引用
刘莉娜,王志强,朱筠..活性维生素D3干预保护2型糖尿病模型大鼠肝脏的作用机制[J].中国组织工程研究,2016,20(5):694-700,7.基金项目
国家自然科学基金(81160116)@@@@ Calcitriol （81160116）
Diabetes Melitus, Type 2 （）
inflammation （）
Tissue Engineering @@@@ the National Natural Science Foundation of China, No.81160116 （）
