中国组织工程研究2016，Vol.20Issue(7)：966-971,6.DOI:10.3969/j.issn.2095-4344.2016.07.007
同种异体和异种来源骨髓间充质干细胞诱导成软骨修复喉软骨缺损
Allogeneicversus heterogeneous bone marrow mesenchymal stem cells for laryngeal cartilage repair
刘艺昌 1周敬2
作者信息
- 1. 南阳市中心医院，河南省南阳市 473009
- 2. 郑州大学第一附属医院，河南省郑州市 450003
- 折叠
摘要
Abstract
BACKGROUND:Because chondrocytes have no regeneration ability, to select suitable seed cels is the primary problem to repair cartilage defects. <br> OBJECTIVE:To investigate the effect of alogeneicversus heterologous bone marrow mesenchymal stem cels (BMSCs) in repairing laryngeal cartilage defects after chondrogenic induction. <br> METHODS:BMSCs from human and rabbits were isolated and cultured. Passage 3 cels were cultured in chondrogenic induction medium containing transforming transforming growth factor beta 1 and bone morphogenetic protein, and then were dropped onto a poly(lactic-co-glycolic acid) (PLGA) scaffold. Thirty New Zealand rabbits were randomly assigned into three groups: blank control group, human BMSCs group, rabbit BMSCs group. Animal models of laryngeal cartilage defects were made in the three groups. After modeling, saline-soaked PLGA scaffold, PLAG scaffold with human BMSCs or with rabbit BMSCs were implanted respectively into the rabbits in the normal blank, human BMSCs and rabbit BMSCs groups. The expression of type II colagen in the larynx and its surrounding tissues was detected by immunohistochemistry at 4 and 8 weeks postoperatively. <br> RESULTS AND CONCLUSION: The animals in each group breathed normaly with no presence of wheezing, and their eating and activity were good. Moreover, there was no purulency or infection in the three groups. At 4 and 8 weeks after operation, the positive rates of type II colagen in the two BMSCs groups were significantly higher than that in the blank control group (P < 0.05). There was no significant difference between two BMSCs groups (P > 0.05). These results show that both alogeneic and heterologous BMSCs have good therapeutic effects on the repair of laryngeal cartilage defects in rabbits.关键词
组织构建/软骨组织工程/软骨缺损/修复/骨髓间充质干细胞/聚乳酸-羟基乙酸共聚物/定向诱导/同种异体/异种分类
医药卫生引用本文复制引用
刘艺昌,周敬..同种异体和异种来源骨髓间充质干细胞诱导成软骨修复喉软骨缺损[J].中国组织工程研究,2016,20(7):966-971,6.
