中国组织工程研究2016，Vol.20Issue(7)：993-999,7.DOI:10.3969/j.issn.2095-4344.2016.07.011
间充质干细胞条件培养液促进肺癌细胞上皮间质转化
Human bone marrow mesenchymal stem cells promote epithelial mesenchymal transition in lung cancer cells
摘要
Abstract
BACKGROUND:The complex relationship between bone marrow mesenchymal stem cels and cancers severely limit the clinical application of mesenchymal stem cels. So it is urgent to study the role of mesenchymal stem cels in tumor growth and metastasis. <br> OBJECTIVE:To explore the effect of human bone marrow mesenchymal stem cels on epithelial mesenchymal transition in non-smal cel lung cancer A549 and PAa cels. <br> METHODS:The A549 and PAa cels were cultured with mesenchymal stem cel supernatant (mesenchymal stem cel conditioned medium, MSCs-CM). The celular morphology was observed under a microscope. The mRNA and protein expression of E-cadherin, N-cadherin, Vimentin, Slug, Snail, and Twist were determined by RT-PCR and western blot. Transwel and wound healing assay were used to detect the change of migration and metastatic ability. <br> RESULTS AND CONCLUSION:Compared with the control group, the celular morphology of experimental group showed mesenchymal-like changes. In response to MSCs-CM, there was decreased E-cadherin but increased N-cadherin, Vimentin and Slug, Snail, Twist at mRNA and protein levels compared with the control group (P < 0.05). The migration and metastatic abilities of the experimental group were also increased. So, human bone marrow mesenchymal stem cels can promote epithelial mesenchymal transition in A549 and PAa cels, and enhance the migration and metastatic abilities of A549 and PAa cels.关键词
组织构建/组织工程/间充质干细胞/肺癌/上皮间质转化/转移/A549细胞/PAa细胞/条件培养/国家自然科学基金分类
医药卫生引用本文复制引用
吴佳斌,王涛,杨伟林,王均洁,肖婕婓,王儒琛,陈振光..间充质干细胞条件培养液促进肺癌细胞上皮间质转化[J].中国组织工程研究,2016,20(7):993-999,7.基金项目
国家自然科学基金(青年科学基金项目)(81101774)；中国博士后科学基金(2014M552218)Funding：the National Natural Science Foundation of China, No.81101774 （青年科学基金项目）
the China Postdoctoral Science Foundation, No.2014M552218 （）
