云南化工2016，Vol.43Issue(2)：17-21,59,6.DOI:10.3969/j.issn.1004-275X.2016.02.005
纳米粒子三明治型传感器的组装及其在 BRCA －1检测中的应用
Nanoparticles -fabricated Sandwich Biosensor for Single -mismatched BRCA -1 Detection
摘要
Abstract
In this study,a sandwich biosensor consisting of PMP (para -magnetic particles)/DNA /Au -NPs (gold nanoparticles)was fabricated.PMPs captured and separated target DNA while Au -NPs modified with oligonucleotide detection sequences played a role in recognition and signal production.There-fore,quantitative information concerning the target analyte was translated into a colorimetric signal,which could be easily and quantitatively measured by low -cost UV -vis spectrophotometric analysis.The results indicated that it was a very simple and economic strategy for detection of single -mismatched DNA strands.关键词
纳米金/纳米磁/三明治型传感器/BRCA -1/基因突变Key words
gold nanoparticles/para -magnetic particles/sandwich biosensor/BRCA -1 detection/single -mismatched分类
信息技术与安全科学引用本文复制引用
展宗瑞,席静,胡久平..纳米粒子三明治型传感器的组装及其在 BRCA －1检测中的应用[J].云南化工,2016,43(2):17-21,59,6.基金项目
甘肃省高等学校科研项目（2014B －124）。 （）
