中国动物检疫2016，Vol.33Issue(2)：29-33,5.
活体动物中大环内脂类残留检测微生物抑制法的建立
Establishment of Microbial Inhibition Method for Lipid Residues Detection in Living Animals
黄晨 1陈本龙 1王乃福 1李卫华2
作者信息
- 1. 天津出入境检验检疫局动植物与食品检测中心，天津 300461
- 2. 中国动物卫生与流行病学中心，青岛 266000
- 折叠
摘要
Abstract
Objective] To establish a detection method for Macrolide Antibiotics in living animals.[Methods]The con-ditions of Microbiological inhibition assay method were optimized, such as selectivity and speciifcity of the Micrococ-cus Luteus CMCC28001, which was a sensitive strain to the test, the sample extraction method, the condition of plate preparation and the detection limit of the procedure.[Results]The procedure was suitable for testing the residue of Mac-rolide Antibiotics(Erythrocin, Tylosin, Spriamycin, Lincomycin, Kitasamycin, Clindamycin). When the inoculum concentration were 10-3(5.5×106CFU/mL)of the Micrococcus Luteus, the inhibition zone diameters were the biggest of the center concentration and the edges were clearly. And the best pH of substrate was 7.5, and the best culture tempera-ture was 30℃.[Conclusions]The microbial inhibition method of Micrococcus Luteus used as an indicator is capable of detecting antibiotic residues in animal sera, simple for operation, and low in cost and has good sensitivity and repeat-ability. This study provides useful supplement for other methods such as color spectrum analysis and immune analysis.关键词
微生物抑制法/藤黄微球菌/大环内脂/残留/活体动物/抗生素Key words
Microbiological inhibition assay method/Micrococcus Luteus/Macrolide/residue/living animals/anti-biotics分类
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黄晨,陈本龙,王乃福,李卫华..活体动物中大环内脂类残留检测微生物抑制法的建立[J].中国动物检疫,2016,33(2):29-33,5.
