中国动物检疫2016，Vol.33Issue(2)：69-72,81,5.
实时荧光RT-PCR鉴别小反刍兽疫病毒疫苗株和野毒株
Detection and Differentiation of Vaccine and Wild-type Strains of PPRV by Duplex Real-time RT-PCR
摘要
Abstract
To differentiate vaccine virus and wild-type peste des petits ruminants virus(PPRV)by duplex real-time RT-PCR,one pair of primers and two TaqMan probes were designed based on the comparison of PPRV genome sequences available in GenBank. The duplex real-time RT-PCR results showed that the detection limits for vaccine strain and wild-type strains were both 10 copies per reaction. The developed assay also showed high ampliifcation efifciency,linearity and speciifcity in discriminating the two PPRV types. A total of 136 clinical samples tested by the conventional RT-PCR were run again by the duplex real-time RT-PCR. The results were conifrmed subsequently by sequencing the positive conventional RT-PCR products. This reliable and rapid duplex real-time RT-PCR assay would be feasible and attractive for diagnostic laboratories,both for clinical purpose in active surveillance and for epidemiological investigations.关键词
小反刍兽疫病毒/谱系IV/Nigeria 75/1疫苗/实时荧光RT-PCRKey words
peste des petits ruminants virus/lineage IV/Nigeria 75/1 vaccine/real-time RT-PCR分类
农业科技引用本文复制引用
李林,吴晓东,刘春菊,王清华,包静月,王志亮..实时荧光RT-PCR鉴别小反刍兽疫病毒疫苗株和野毒株[J].中国动物检疫,2016,33(2):69-72,81,5.基金项目
公益性行业（农业）科研专项 （）
