中国比较医学杂志2016，Vol.26Issue(3)：1-5,5.DOI:10.3969.j.issn.1671-7856.2016.03.001
尾吊小鼠感染空间诱变大肠杆菌炎症反应增强
Increased inflammatory reaction in tail-suspension mice infected by E.coli in spaceglight
摘要
Abstract
Objective To observe the changes of inflammatory reaction in tail-suspension mice after infected by E.coli in spaceflight.Methods 40 C57BL/6 mice were randomly divided into four groups:control group(Con), control+E.coli T1-13 group(Con+T1-13), tail suspension group(TS), tail suspension+E.coli T1-13 group(TS+T1-13).The inflammatory cytokines TNF-α, IL-1βand IL-6 production in plasma and mRNA level in intestinal tissue were detected by ELISA and RT-qPCR, and HE staining was used to represent the morphology changes in small intestine tissue.Results Compared with the control group, the expression of inflammatory cytokines in plasma and intestinal tissue of all experimental groups were increased, and the TS+T1-13 group was most significant( P<0.01 or P<0.001) .HE staining showed that the small intestine mucosa in the experimental groups were damaged in different degrees, and the damage of TS+T1-13 group was most serious.Conclusions The E.coli from spaceflight increased significantly the expression of inflammatory cytokines in plasma and intestinal tissue from infected tail-suspension mice, and brought more serious damages to the small intestinal mucosal barrier, which suggested that the inflammatory reaction would be increased in tail-suspension mice infected by E.coli from spaceflight.关键词
空间诱变大肠杆菌/模拟失重/炎症因子/肠道组织Key words
Space mutation E.coli/Simulated microgravity/Inflammatory cytokines/Intestinal tissue分类
医药卫生引用本文复制引用
姚静,程江,裴雪枫,王静宇,王俊锋,张学林,刘长庭,袁明..尾吊小鼠感染空间诱变大肠杆菌炎症反应增强[J].中国比较医学杂志,2016,26(3):1-5,5.基金项目
国家重点基础研究发展计划(973 计划)(2014CB744404). （973 计划）
