中国比较医学杂志2016，Vol.26Issue(3)：15-18,4.DOI:10.3969.j.issn.1671-7856.2016.03.004
高脂饮食结合STZ诱导妊娠期糖尿病小鼠模型的建立
High fat diet combined with streptozotocin-induced gestational diabetes mellitus on mice
摘要
Abstract
Objective To explore a gestational insulin resistance mice model establishing method, which is steady, reliable and similar to human being.Method Female KM mice at 5 weeks were randomly divided into high fat diet group(n=30) and normal diet group(n=30).High fat diet group was exposed to high diet for 4 weeks, and then male and female were mated ( n:n=1:1 ) Then the pregnancy mice were intraperitoneal injected with 30 mg/kg/d STZ during the first 3 days, the control group was injected with equal dosage of citrate buffer solution( 0.1 mol/L, pH=4.2) for 3 days.Their blood glucose, body weight, the amount of food intake and water intake were recorded at 3,7,14 and 19 d of the GDM model respectively.The serum INS, ADP, LEP, CRP were measured by ELISA.Results We successfully made the GDM mice model, and the pregnant mice showed significant signs of polyuria, polydipsia, hyperphagia and weight loss compared to the control model(P <0.01).The blood glucose of GDM mice >11.1mmol/L,the serum cytokines (INS(1.50 ±0.25)Mu/L, ADP(0.65 ±0.13)μg/L, LEP(1.60 ±0.12)μg /L, CRP(37.54 ±4.70)μg /L ) of GDM mice were significant difference compared to the control model(P <0.01).Postpartum, the GDM mice blood glucose returned to the normal level.Conclusion Gestational diabetes mellitus mice model can be successfully developed by high fat diet with low dose STZ and three times induce, which preferable mimics the characteristic of gestational pathologic insulin resistance in human beings.关键词
妊娠期糖尿病/高脂饮食/链脲佐菌素/模型/小鼠Key words
Gestational diabetes mellitus/High fat diet/Streptozotocin/Model/Mice分类
医药卫生引用本文复制引用
陈琳,黄小丽,杨亚旭,周美佳,郝臻凤,孔桂美,胡荣,卜平..高脂饮食结合STZ诱导妊娠期糖尿病小鼠模型的建立[J].中国比较医学杂志,2016,26(3):15-18,4.基金项目
国家自然科学基金资助项目(81272537 （）
30801497) （）
江苏省高校自然科学基金资助项目(11KJB360010) （11KJB360010）
扬州大学"新世纪人才工程"资助(2012). （2012）
