中国比较医学杂志2016，Vol.26Issue(3)：24-28,5.DOI:10.3969.j.issn.1671-7856.2016.03.006
莫诺苷对脑缺血再灌注大鼠皮层肝细胞生长因子及血管性血友病因子表达的影响
Effects of morroniside on the expression of the HGF and vWF in peri-infarct cortex after cerebral ischemia-reperfusion in rats
摘要
Abstract
Objective To study the effects of morroniside on the expression of the HGF and vWF of the ischemic ipsilateral cortex 7 days after ischemia reperfusion.Methods 25 male Sprague-Dawley rats were subjected to MCAO model with modified Zea Longa's method, then randomly divided into sham group (n=5), ischmia group (n=5), and morroniside groups ( low, medium, and high dosage groups, n=5 ) .Morroniside were then administered intragastrically once a day at dose of 30 mg/kg, 90 mg/kg and 270 mg/kg after operation.The expression of HGF and vWF of the ischemic ipsilateral cortex were detected by western blotting and Immunofluorescence 7 days after ischemia-reperfusion. Results Compared with the sham group, the expression of HGF in ischemia group increased significantly ( P<0.01 ) 7 days after MCAO.Compared with the ischemia group, the expression of HGF in the morroniside groups (30 mg/kg,90mg/kg,270 mg/kg) showed significant increased ( P<0.05, P<0.01, P<0.001).Compared with the sham group, the expression of vWF in ischemia group increased significantly ( P <0.001).Compared with the ischemia group, the expression of vWF in the morroniside groups (90 mg/kg, 270 mg/kg ) showed significant increased (P <0.01, P <0.001) .Conclusion Morroniside could increase the expression of HGF and vWF in the ischemic ipsilateral cortex, romoting the process of angiogenesis in focal cerebral ischemia rat.关键词
莫诺苷/脑缺血再灌注/免疫印迹/免疫荧光/HGF/vWFKey words
Morroniside/Cerebral ischemia-reperfusion/Western blotting/Immunofluorescence/HGF/VWF分类
医药卫生引用本文复制引用
魏仁平,孙芳玲,刘婷婷,程华,艾厚喜,郭德玉,田欣,祝自新,王宇峰..莫诺苷对脑缺血再灌注大鼠皮层肝细胞生长因子及血管性血友病因子表达的影响[J].中国比较医学杂志,2016,26(3):24-28,5.基金项目
国家自然科学基金(81373994 （）
81173575) （）
"重大新药创制"科技重大专项(2012ZX09102201-106). （2012ZX09102201-106）
