中国比较医学杂志2016，Vol.26Issue(3)：29-34,6.DOI:10.3969.j.issn.1671-7856.2016.03.007
长爪沙鼠肝星状细胞的分离培养与鉴定
Establishment of a primary culture protocol of Mongolian gerbil hepatic stellate cells
摘要
Abstract
Objective To investigate the method to isolate and culture hepatic stellate cells ( HSCs) for studying the cellular mechanisms of hepatic frbrosis.Methods HSCs were isolated by nycodenz density gradient centrifugation after the hepatocytes obtained from adult male gebils were digested with pronase, collagenase and DNase, infused via portal vein.The cell viability was determined by trypan blue exclusion test.The purity of HSCs was identified by detectingα-SMA, desmin immunohistochemical staining.Results The yield rate of HSCs was 0.5~1 ×107 per gerbil liver, and the cell viability was more than 90%.The percentage ofα-SMA-positive cells was more than 75%after 3 days primary culture and almost 100% cells were α-SMA and desmin positive in passage culture.Conclusion The successful protocol of primary culture of Mongolian gerbil HSC provide a technical support for research of relevant liver diseases and drug development in the future.关键词
长爪沙鼠/肝星状细胞/细胞分离/原代培养Key words
Mongolian gerbil/Hepatic stellate cell/Cell isolation/Primary culture分类
医药卫生引用本文复制引用
楼琦,李巍,石巧娟,卢领群,郭红刚,杜江涛,萨晓婴..长爪沙鼠肝星状细胞的分离培养与鉴定[J].中国比较医学杂志,2016,26(3):29-34,6.基金项目
国家自然科学基金(31301933) （31301933）
浙江省科技计划项目(2013C37012, 2013C37013, 2014C27013). （2013C37012, 2013C37013, 2014C27013）
