中国比较医学杂志2016，Vol.26Issue(3)：46-51,6.DOI:10.3969.j.issn.1671-7856.2016.03.010
肺腺癌A549绿色荧光裸鼠模型中肿瘤血管内皮细胞来源初探
Research on the source of endothelial cells in tumor vessels by A 549 tumor model with GFP nude mouse
方天 1胡若愚 2胡文娟 1刘彪 1尤金炜 1恽时锋1
作者信息
- 1. 南京军区南京总医院比较医学科,南京 210002
- 2. 东南大学附属中大医院胸心外科,南京 210002
- 折叠
摘要
Abstract
Objective To explore the source of endothelial cells in tumor vessels by A 549 tumor model with GFP nude mouse.Methods To establish the A 549 lung cancer models with GFP nude mice, expression of CD 31 was determined by immunofluorescence to label tumor vessels; to observe and take a picture of the tumor frozen section by confocal microscopy and invert microscope;expression of GFP in tumor vessels was determined by immunohistochemistry. Result The results of immunofluorescence showed:Tumor interstitial vascular endothelial cells or endothelial cells clusters and micro-vascular lumen size and shape are clearly visible by immunofluorescence, and part of vessels with no obvious lumen or irregular lumen.We can see green fluorescent in tumor cells of tumor tissue and endothelial cells which form of tumor vessels.The results of immunohistochemistry showed: expression of GFP was determined in cytoplasm of tumor stromal cells and endothelial cells in tumor vessels.Conclusion The endothelial cells which formed tumor neovessels that derived from GFP nude mice partly and the other part derived from tumor cells.关键词
肺腺癌A549/血管生成/内皮细胞/肿瘤干细胞Key words
A549/Vascularization/Endothelial cells/Carcinoma stem cells分类
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方天,胡若愚,胡文娟,刘彪,尤金炜,恽时锋..肺腺癌A549绿色荧光裸鼠模型中肿瘤血管内皮细胞来源初探[J].中国比较医学杂志,2016,26(3):46-51,6.
