中国比较医学杂志2016，Vol.26Issue(3)：52-57,6.DOI:10.3969.j.issn.1671-7856.2016.03.011
Nrf2抗氧化通路在CCl4所致大鼠急性肝损伤中的保护作用
Effect of Nrf2 signal pathway on acute hepatotoxicity induced by CCl4 in rat
周清平 1蒋孝华 1符小波2
作者信息
- 1. 南华大学附一医院感染科,湖南衡阳 421001
- 2. 湖南耒阳市人民医院神经内科,湖南耒阳 421800
- 折叠
摘要
Abstract
Objective To determine the effect of Nuclear factor-erythroid 2-related factor 2 ( Nrf2 ) on acute hephrotoxicity induced by CCl4 in male rat.Methods 20 male Wistar rats were randomly divided into control group and CCl4 group, 10 rats in each group, another 10 male Wistar rats were transgenic rats microinjection through the carrier, obtained the Nrf2-tk gene integration and specific transgenic rats, as the CCl4 +Nrf2 integration group.The groups was given 1%polysorbate 80 for 4 days,Then the CCl4 and CCl4 +Nrf2 integration group were intraperitoneally injected with a single dose of CCl47.5 mg· kg-1 and were killed 24 h after CCl4 injection.The serum chemical parameters including asparate aminotransferase ( AST) ,alanine aminotransferase ( ALT) and lactate dehydrogenase ( LDH) were measured.Also malonaldehyde ( MDA) ,glutathione ( GSH) ,oxidized glutathione ( GSSG) levels in the liver as well as glutathione ( GSH)/oxidized glutathione ( GSSG ) ratios were detected. Histopathologic changes in the liver were examined. Results F1generation TK transgenic rats in liver and testis and other tissues and organs were not detected the transcription of Nrf2-tk, indicating that Nrf2-tk expression in tissues is specific good.Nrf2 significantly reduced serum AST, ALT and LDH levels in a dose-dependent manner.The results of MDA levels and GSH/GSSG ratios in liver and kidney showed that Nrf2 reduced CCl4-induced hepatic lipid peroxidation,and ameliorated glutathione depletion.The histopathologic results showed that Nrf2 restrained liver and kidney damage induced by CCl4 .Conclusion Nrf2 can effectively protect male rat from acute hepatotoxicity and nephrotoxicity induced by CCl4 .关键词
Nrf2/转基因大鼠/四氯化碳/急性肝损伤Key words
Nuclear factor-erythroid 2-related factor 2 ( Nrf2 )/Transgenic rat models/CCl4/Acute hepatotoxicity induced分类
医药卫生引用本文复制引用
周清平,蒋孝华,符小波..Nrf2抗氧化通路在CCl4所致大鼠急性肝损伤中的保护作用[J].中国比较医学杂志,2016,26(3):52-57,6.
