中国比较医学杂志2016，Vol.26Issue(3)：75-80,6.DOI:10.3969.j.issn.1671-7856.2016.03.015
H-1细小病毒抗体ELISA检测方法的建立与应用
Establishment and application of ELISA method for H-1 parvovirus
摘要
Abstract
Objective To establish ELISA method for H-1 parvovirus, and to apply it in detection.Method Cultured the H-1 parvovirus in rat glioma cell line C6, prepared the viral antigen for coating.Used the purified viral antigen to establish the ELISA method, and compared the ELISA method with the ELISA kit from XpressBio company. Then applied the ELISA method in detection of 35 rat serums.Results The positive serum which be diluted to 1280 can be detected by the ELISA method, there have not cross reaction with positive serum of CPV, MVM and PPV, but there has cross reaction with KRV.35 pieces of rat serums were detected by the ELISA method, they were all negative, the results were consistent with the kit from XpressBio company.Conclusions The sensitivity and species specificity of the ELISA method for H-1 parvovirus were suitable, the method can be used in detection of H-1 parvovirus in rat serum.关键词
H-1细小病毒/ELISA/抗体检测Key words
H-1 parvovirus/ELISA/Detection of antibody分类
医药卫生引用本文复制引用
付瑞,李晓波,王淑菁,王吉,卫礼,巩薇,岳秉飞,贺争鸣..H-1细小病毒抗体ELISA检测方法的建立与应用[J].中国比较医学杂志,2016,26(3):75-80,6.基金项目
国家科技支撑计划"实验用动物病原分子生物学快速检测新技术研究与应用"(2015BAI07B02). （2015BAI07B02）
