中国比较医学杂志2016，Vol.26Issue(3)：86-88,3.DOI:10.3969.j.issn.1671-7856.2016.03.017
微核试验的改进
Experience exchange in vitro micronucleus assay
马宝良 1鲁克庆2
作者信息
- 1. 兰州大学第二医院泌尿外科研究所,甘肃省泌尿系统疾病研究重点实验室,兰州 730030
- 2. 甘肃省泌尿系统疾病临床医学中心,兰州 730030
- 折叠
摘要
Abstract
Objective To compare the conventional marrow collection with new marrow collection, in the number of cell, other tissues pollution and the background of the smear, providing the reference for future micronucleus test. Methods The mice was enthanasia and the sternums were taken.One group, using the conventional method of marrow collection, squeezing the marrow to the slide with fetal bovine serum;the other group, using 1-mL injector extracting fetal bovine serum 100μL, injecting into mice sternums and rushing out the bone marrow for circle smear.Results Two methods can meet the requirement of test, but the new marrow collection can acquire more number of cells, less the tissues pollution and more clear in the background of smear.Conclusions Comparing with the conventional marrow collection, the new method has more superiority to simplify the next cell counting.关键词
骨髓采集/胎牛血清/细胞/小鼠Key words
Marrow collection/Fetal bovine serum/Cell/Mice分类
医药卫生引用本文复制引用
马宝良,鲁克庆..微核试验的改进[J].中国比较医学杂志,2016,26(3):86-88,3.
