中国现代医学杂志2016，Vol.26Issue(6)：1-5,5.DOI:10.3969/j.issn.1005-8982.2016.06.001
腺病毒Smad7转染对阿霉素肾病大鼠蛋白尿的影响
Effect of adenovirus-mediated Smad7 gene transfer on proteinuria of Adriamycin-induced nephropathy rat model
冯笑琪 1林娜娜 1谢燕媚 1纪泽泉1
作者信息
- 1. 广州医科大学附属第二医院，广东 广州 510260
- 折叠
摘要
Abstract
Ojective To investigate the effect of overexpression of Smad7 on proteinuria and renal pathology in the Adriamycin-induced nephropathy (ADN) rat model. Methods ADN model was established by single tail intravenous injection of Adriamycin (ADR). Smad7 gene was transferred by means of infusing a recombinant adenovirus into the left renal artery. Recombinant adenovirus-Smad7 (Ad-Smad7) was detected under fluorescence microscope. HE staining was used to examine renal pathological changes of each group. Pyrogallol red colorimetry was used to detect 24-h urinary protein. Results SD rats received a single intravenous injection of ADR (6.5 mg/kg), which constructed the ADN model. The pathology was similar to minimal change nephropathy at the fourth weekend. Unilateral renal artery infusion was efficient to transfer Ad-Smad7 to the target kidney. Urinary protein of the Ad-Smad7 group (38.4 ± 6.0) decreased compared to the ADR group (69.58 ± 10.63) at the fourth weekend ( < 0.05). Smad7 was mainly expressed in renal tubular epithelia in the Ad-Smad7 group, scarcely seen in glomeruli. The pathological changes of the Ad-Smad7 group were mild, while the changes of the Ad-GFP and ADR groups were similar to focal segmental glomerulosclerosis. Conclusions Ad-Smad7 can be transferred to the target kidney through unilateral renal artery infusion, and overexpression of Smad7 can ameliorate proteinuria and pathological changes of Adriamycin-induced nephropathy.关键词
重组腺病毒/Smad 7/肾病/蛋白尿Key words
recombinant adenovirus/Smad7/nephropathy/proteinuria分类
医药卫生引用本文复制引用
冯笑琪,林娜娜,谢燕媚,纪泽泉..腺病毒Smad7转染对阿霉素肾病大鼠蛋白尿的影响[J].中国现代医学杂志,2016,26(6):1-5,5.
