中国现代医学杂志2016，Vol.26Issue(6)：6-10,5.DOI:10.3969/j.issn.1005-8982.2016.06.002
I型钠氢交换蛋白调节细胞内酸碱平衡对胃癌细胞迁移、侵袭的影响
Effect of NHE1-regulated intracellular pH on invasion and migration of gastric cancer cells
摘要
Abstract
objective To observe the effect of Na+/H+ exchanger 1 (NHE1)-regulated intracellular pH on the invasion and migration of gastric cancer cell line SGC-7901. Methods The gastric cancer cell line SGC -7901 was treated with the NHE1 specific inhibitor 5-(N-ethyl-N-isopropyl) Amiloride (EIPA), the intracellular pH of SGC-7901 was measured using confocal fluorescence microscope. The migration of SGC-7901 cells was examined by scratch test. The invasion of SGC-7901 cells was examined by transwell chamber invasive test. Results The intracellular pH in the control group was (7.2 ± 0.12), it significantly reduced to (7.01 ± 0.23) in the 5-μmol EIPA group. EIPA of different concentrations (5 μmol, 10 μmol and 20 μmol) could inhibit the invasion and migration of SGC-7901 cells in a dose-dependent manner. Conclusions NHE1 plays an important role in the regulation of intracellular pH. Blockage of NHE1 could restrain the invasion and migration of gastric cancer cells.关键词
NHE1/胃癌/细胞迁移/pH/细胞侵袭Key words
NHE1/gastric cancer/cell migration/pH/cell invasion分类
医药卫生引用本文复制引用
谢睿,王海波,牟海军,徐靖宇..I型钠氢交换蛋白调节细胞内酸碱平衡对胃癌细胞迁移、侵袭的影响[J].中国现代医学杂志,2016,26(6):6-10,5.基金项目
贵州省卫生计生委科学技术基金项目 （）
