中国现代医学杂志2016，Vol.26Issue(6)：11-14,4.DOI:10.3969/j.issn.1005-8982.2016.06.003
microRNA-10b及靶基因HOXD10在肝癌细胞中的表达及侵袭能力的影响
Expression of miR-10b and target gene HOXD10 in liver cancer cell lines and its impact on tumor invasion
摘要
Abstract
Objective To investigate the expression of miR-10b and its targeted gene HOXD10 in hepatic carci-noma cell lines and its influence on tumor invasion. Methods Basic expression of HOXD10 gene in the three hepa-tic carcinoma cell lines (Huh7, HepG2 and SMMC-7721) was determined. Mature mimics of miR-10b were chemi-cally synthesized and transiently transfected into higher HOXD10-expression cell line HepG2 cells via lipofectamine 2000. Cells were also transfected with empty vectors and unrelated fragment to serve as negative controls. The inva-sion of HepG2 cells was investigated by Transwell assay. The expressions of HOXD10 were detected by RT-PCR and Western blot. Results The expression levels of gene and protein at 2- ΔΔCT were (1.24 ± 0.23) and (1.00 ± 0.02) without significant difference ( >0.05). In protein analysis of HepG2 cells, gray value was calculated to be 653.492 and 1968.742 respectively, and protein expression levels were reduced. Target gene HOXD10 was over-expressed in HepG2 cells and the ability of tumor invasion increased. Conclusions Synthesized miRNA-10b can regulate the expression of target gene HOXD10 and promote the invasion of the hepatic carcinoma cells.关键词
肝癌细胞/microRNA- 10b/HOXD10/侵袭Key words
hepatic carcinoma cell/miRNA-10b/HOXD10/invasion分类
医药卫生引用本文复制引用
熊建宁,胡立春,王亚兵,段雪飞..microRNA-10b及靶基因HOXD10在肝癌细胞中的表达及侵袭能力的影响[J].中国现代医学杂志,2016,26(6):11-14,4.基金项目
广东省佛山市卫生和计划局医学科研课题 （）
