中国现代医学杂志2016，Vol.26Issue(6)：15-18,4.DOI:10.3969/j.issn.1005-8982.2016.06.004
胰岛素样生长因子结合蛋白7过表达对人乳腺癌细胞系-7增殖的影响及其机制研究
Effect of IGFBP7 overexpression on proliferation of breast cancer cell line MCF-7
魏丽瑶 1岳春燕 1彭健1
作者信息
- 1. 中南大学湘雅医院 普外科，湖南 长沙 410008
- 折叠
摘要
Abstract
Objective To investigate the effect of insulin-like growth factor binding protein 7 (IGFBP7) on prolife-proliferation of human breast cancer cell line MCF-7 and its mechanism. Methods Plasmid pIRES2-ZsGreen1-IGFBP7 or empty plasmid pIRES2-ZsGreen1 was transfected into MCF-7 cells using LipofectamineTM 2000 and the cell transfection efficiency was examined by fluorescence microscopy. Real-time fluorescent quantitative PCR (RTFQPCR) was used to quantify the expression of IGFBP7. MTT was performed to evaluate the effect of IGFBP7 on proliferation and apoptosis of MCF-7 cells at 24, 48 and 72 hours after transfection. The expression of relevant proteins of AKT/mTOR signaling pathway was analyzed by Western blot. Results The IGFBP7 mRNA level increased significantly after IGFBP7 transfection. Compared with the controls, cell proliferation noticeably decreased in the IGFBP7- transfected group. Western bolt analysis indicated that the AKT phosphorylation was down-regulated, and the mTOR level dropped markedly ( < 0.05). Conclusions Overexpression of IGFBP7 could down-regulate the proliferation of MCF-7 cells, possibly via inhibiting the AKT/mTOR signaling pathway.关键词
实时荧光定量聚合酶链反应/胰岛素样生长因子结合蛋白7/增殖/蛋白激酶BKey words
real- time quantitative polymerase chain reaction/insulin- like growth factor binding protein 7/proliferation/protein kinase B分类
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魏丽瑶,岳春燕,彭健..胰岛素样生长因子结合蛋白7过表达对人乳腺癌细胞系-7增殖的影响及其机制研究[J].中国现代医学杂志,2016,26(6):15-18,4.
