中国现代医学杂志2016，Vol.26Issue(7)：4-8,5.DOI:10.3969/j.issn.1005-8982.2016.07.002
兔软骨细胞的分离及其与Ⅰ/Ⅱ型复合胶原膜共培养实验研究
Isolation of rabbit chondrocytes and their co-culture with type Ⅰ/Ⅱ collagen membrane
摘要
Abstract
Objective To explore the method of isolating rabbit chondrocytes and observe their bioactivity when co-cultured with type Ⅰ/Ⅱ collagen membrane. Methods Articular cartilage was taken under aespetic conditions after the New Zealand rabbits at 4 weeks of age were sacrificed. Chondrocytes were di_gested by trypsin and type Ⅱ collagenase. The features of the chondrocytes were observed under inverted phase contrast microscope. The slides of cells were stained by toluidine blue. The third-generation rabbit chondrocytes were co-cultured with type Ⅰ/Ⅱ collagen membrane. The growth of the chondrocytes on typeⅠ/Ⅱ collagen membrane was observed by the method of MTT, test of glycosaminoglycan and scaning electron microscopy. Results The original-generation chondrocytes were oval in the beginning, then they were triangle or polygan. The chondrocytes could be stained by toluidine blue. They grew well on the type Ⅰ/Ⅱ collagen membrane. Conclusions A large number of chondrocytes can be obtained by two-enzyme method. Chondro_cytes can amplify normally on the type Ⅰ/Ⅱ collagen membrane, which can be demonstrated by MTT method and scaning electron microscopy.关键词
软骨细胞/复合胶原膜/胰蛋白酶/Ⅱ型胶原酶/细胞活性/软骨缺损Key words
chondrocyte/type Ⅰ/Ⅱ collagen membrane/trypsin/type Ⅱ collagenase/co-culture/bioactivity分类
医药卫生引用本文复制引用
丛波,张加廷,韩龙,丁一,徐训安,姚旺林,张仲文..兔软骨细胞的分离及其与Ⅰ/Ⅱ型复合胶原膜共培养实验研究[J].中国现代医学杂志,2016,26(7):4-8,5.基金项目
首都临床特色应用研究项目（NoZ161100000516013）；武警总医院一类课题 （）
