中国现代医学杂志2016，Vol.26Issue(7)：9-14,6.DOI:10.3969/j.issn.1005-8982.2016.07.003
特异性抑制转化生长因子β激活激酶1活性对妊娠期糖尿病孕妇外周血单核细胞炎症通路的影响
Influence of specifical inhibition of TAK1 activity in inflammatory pathway of mononuclear cells in peripheral blood of pregnant women with gestational diabetes mellitus
摘要
Abstract
Objective To investigate the influence of changes of TGF-β-activated kinase 1 (TAK1) activity in the inflammatory pathway of mononuclear cells in the peripheral blood of pregnant women with gestational diabetes mellitus (GDM). Methods Totally 30 GDM pregnant women and 30 normal pregnant women were selected. Peripheral venous blood was drawn, and mononuclear cells and plasma were separated. They were divided into lipopolysaccharide (LPS)-stimulation group (L group), LPS and TAK1-inhibitor group (LT group), TAK1-inhibitor group (T group) and control group (W group). After culture, Western blot was used to detect the expressions of TAK1 and NF-κB, and ELISA was used to detect the plasma concentration of LPS and the expression levels of inflammatory factors (IL-1, IL-10 and TNF-α). The differences between GDM and normal pregnant women were compared and the effect of TAK1 was explored. Results Compared with the normal pregnant women, the plasma LPS concentration, the expression levels of inflammatory factors (IL-1, IL-10 and TNF-α), TAK1 and NF-κB increased in the GDM pregnant women ( <0.05). Compared with oth_er groups, the expression levels of TAK1, NF-κB and inflammatory factors (IL-1, IL-10 and TNF-α) signifi_cantly increased in the L group ( <0.05). In the normal pregnant women, the expression levels of NF-κB had no statistical differences ( >0.05) among the T group, the LT group and the W group; no obvious ex_pression of TAK1 was detected in the LT group or the T group while a small amount of TAK1 expression was detected in the W group; compared with the T group, the expression levels of inflammatory factors (TNF-α, IL-1 and IL-10) significantly increased in the LT group ( <0.05). In the GDM pregnant women, the LT group, the T group and the W group had no statistical differences in the expression levels of TAK1 or NF-κB ( >0.05); compared with the T group, the expression levels of inflammatory factors (TNF-α, IL-1 and IL-10) significantly increased in the LT group ( <0.05). The Pearson correlation analysis showed that the ex_pression levels of TAK1 and NF-κB were positively correlated with inflammatory factors (IL-1, IL-10 and TNF-α) ( < 0.05). Conclusions TAK1 may be involved in the formation of the inflammatory pathway of mononuclear cells in the peripheral blood of GDM pregnant women. Inhibition of TAK1 activity can down-reg_ulate the expressions of downstream key mediators such as NF-κB and inflammatory factors.关键词
妊娠期糖尿病/炎症通路/脂多糖/转化生长因子β激活激酶1/核转录因子- κB/炎症因子Key words
gestational diabetes mellitus/inflammatory pathway/lipopolysaccharide/transforming growth factor (TGF)-β-activated kinase 1/NF-κB/inflammatory factor分类
医药卫生引用本文复制引用
高传龙,袁静,李琴,杨琴,李松,周培,丛林..特异性抑制转化生长因子β激活激酶1活性对妊娠期糖尿病孕妇外周血单核细胞炎症通路的影响[J].中国现代医学杂志,2016,26(7):9-14,6.基金项目
安徽省自然科学基金 （）
