中国药房2016，Vol.27Issue(8)：1044-1047,4.DOI:10.6039/j.issn.1001-0408.2016.08.11
LC-MS/MS法测定人血浆中卡马西平的浓度及其在室间质评中的应用
Application of LC-MS/MS Method for the Determination of Carbamazepine in Human Plasma and Exter-nal Quality Assessment
摘要
Abstract
OBJECTIVE:To establish and validate the method for the determination of carbamazepine (CBZ) in human plas-ma,and to apply the method for external quality assessment. METHODS:After precipitated with acetonitrile,the plasma sample was determined by LC-MS/MS. Using loratadine as internal standard,the determination was performed on Kromasil C18 column with mobile phase consisted of water (containing 0.1% formic acid)-acetonitrile (gradient elution) at flow rate of 0.6 ml/min and column temperature of 40 ℃. The ion transitions under MRM mode by ESI+ ionization were performed at m/z 237.1→194.0 and m/z 383.1→267.0 for CBZ and internal standard,respectively. RESULTS:The linear range of CBZ were 5-1 000 ng/ml (r>0.998). The limit of quantitation was 5 ng/ml. RSDs of inter-day and intra-day were 1.00%-6.42%;relative deviation were -6.93%-0.32%. The external quality assessment of 5 samples were 679.0,475.0,104.0,29.2 and 26.2 ng/ml,respectively. The pass rate of assess-ment result was 100%. CONCLUSIONS:The method is sensitive,accurate and specific. The method is applicable for the plasma concentration determination and external quality assessment of CBZ.关键词
液相色谱-串联质谱法/卡马西平/血药浓度/室间质评Key words
LC-MS/MS/Carbamazepine/Plasma concentration/External quality assessment分类
医药卫生引用本文复制引用
梅升辉,杨莉,冯卫星,赵志刚,朱乐亭..LC-MS/MS法测定人血浆中卡马西平的浓度及其在室间质评中的应用[J].中国药房,2016,27(8):1044-1047,4.基金项目
Δ基金项目国家自然科学基金资助项目（No.81301118）；多肽及小分子药物北京市重点实验室开放课题 （）
