中国药房2016，Vol.27Issue(12)：1655-1658,4.DOI:10.6039/j.issn.1001-0408.2016.12.24
一测多评法同时测定苏子油胶丸中5种不饱和脂肪酸的含量
Simultaneous Determination of 5 Unsaturated Fatty Acids in Perilla Oil Soft Capsule by Quantitative Analysis of Multi-components by Single Maker
摘要
Abstract
OBJECTIVE:To establish a method for the simultaneous determination of 5 unsaturated fatty acids in Perilla oil cap-sule. METHODS:With the reference material of α-linolenic acid methyl ester,GC was used to determine and calculate the relative correction factors of α-linolenic acid methyl ester with methyl palmitate,methyl stearate,methyl oleate and linoleic acid methyl es-ter,and the correction factors were used to calculate the contents of 5 unsaturated fatty acids;the column was Agilent Innowax cap-illary column,the detector was FID,the inlet temperature was 230 ℃,the detector temperature was 250 ℃,the gas flow rate was 20 ml/min(nitrogen),40 ml/min(hydrogen)and 350 ml/min(air),split ratio was 30 to 1,the column temperature was 190 ℃, and injection volume was 1 μl. RESULTS:The linear range was 0.018-0.792 μg(r=0.9994)for methyl palmitate,0.0016-0.0176μg(r=0.9993)for methyl stearate,0.0056-0.2464 μg(r=0.9999)for methyl oleate,0.003-0.132 μg(r=0.9990)for linoleic acid methyl ester and 0.018-0.792 μg(r=0.9998) for α-linolenic acid methyl ester;RSDs of precision,stability and reproducibility tests were lower than 5%;recoveries were 98.990%-101.70%(RSD=0.720%,n=6) for methyl palmitate,99.599%-100.699%(RSD=0.368%,n=6) for methyl stearate,98.996%-101.680%(RSD=1.240%,n=6) for methyl oleate,99.813%-100.963%(RSD=0.434%,n=6)for linoleic acid methyl ester and 97.185%-99.602%(RSD=0.874%,n=6)for α-linolenic acid methyl es-ter. CONCLUSIONS:The method is simple and stable with good reproducibility,and can be used for the simultaneous determina-tion of methyl palmitate,methyl stearate,methyl oleate,linoleic acid methyl ester,α-linolenic acid methyl ester in Perilla oil cap-sule.关键词
一测多评法/相对校正因子/苏子油胶丸/气相色谱法Key words
Quantitative analysis of multi-components by single marker/Relative correction factor/Perilla oil capsule/GC分类
医药卫生引用本文复制引用
伍静玲,仲海洁,胡军华,于桂芳,黄文哲,王振中,肖伟..一测多评法同时测定苏子油胶丸中5种不饱和脂肪酸的含量[J].中国药房,2016,27(12):1655-1658,4.基金项目
"重大新药创制"科技重大专项(No.2013ZX09402203) （No.2013ZX09402203）
