中国药理学通报2016，Vol.32Issue(4)：480-483,4.DOI:10.3969/j.issn.1001-1978.2016.04.008
三叶青黄酮抗肺癌作用研究
Inhibitory effects of Radix Tetrastigma Hemsleyani Flavone on growth and invasion of lung carcinoma cells
摘要
Abstract
Aim To study the effect of Radix Tetrastig-ma Hemsleyani Flavone ( RTHF ) on the proliferation and invasion in lung carcinoma A549 cells as well as the possible mechanisms underlying these processes. Methods A549 cells were treated with different con-centrations of RTHF for different time. MTT assay and colone formation assay were used to detect the ability of cell proliferation. Wound healing methods and tran-swell chamber assay were adopted to determine cell mi-gration and invasion. Western blotting assay was used to detect the expression of metastasis-related proteins MMP-2 , MMP-9 , and TIMP-2 . Results RTHF obvi-ously suppressed the proliferation of A549 cells in a dose-and time-dependent manner. Microscope found an apparent decrease of cells in the denude zone of cell migration and transwell testing results show that the treatment of invasion was significantly lower than the proportion in the control group ( P <0. 01 ) . The pro-tein expressions of MMP-2 and MMP-9 were down-reg-ulated and that of TIMP-2 was up-regulated in a dose-dependent manner. Conclusion RTHF inhibits the growth and invasion of lung carcinoma A549 cells, which might be achieved by down-regulating the ex-pressions of MMP-2 and MMP-9 protein.关键词
三叶青/抑制/A549细胞/增殖/侵袭转移/MMPKey words
Radix Tetrastigma Hemsleyani/inhibi-tion/A549 cell/proliferation/metastasis/MMP分类
医药卫生引用本文复制引用
钟良瑞,林霜,魏克民..三叶青黄酮抗肺癌作用研究[J].中国药理学通报,2016,32(4):480-483,4.基金项目
国家自然科学基金资助项目( No 81541084) （ No 81541084）
浙江省自然科学基金资助项目(No LQ15H290006) （No LQ15H290006）
