中国药理学通报2016，Vol.32Issue(4)：484-488,489,6.DOI:10.3969/j.issn.1001-1978.2016.04.009
羧甲基茯苓多糖对氟尿嘧啶致肠炎小鼠的保护作用
Protective effects of CMP on 5-Fu-induced intestinal mucositis of mice
摘要
Abstract
Aim To evaluate the mucosal-protective effects of carboxymethylpachyman( CMP) on Fluorou-racil(5-Fu)-induced mice intestinal mucositis and ex-plore its mechanisms. Methods ICR mice were as-signed randomly to four groups:normal group( n=8;re-ceiving pure water orally for 14 d) ,CMP group( n=8;200 mg·kg-1 CMP for 14 d orally),5-Fu group(n=8;25 mg·kg-1 5-Fu for 7 d,intraperitoneally( i. p. ) , and CMP+5-Fu group( n=8;200 mg·kg-1 CMP for 14 d orally and 25 mg·kg-1 5-Fu for 7 d,i. p. ). At day 14the mice were sacrificed. The intestinal propel-ling rate and the colon length were measured. ROS, GSH and IL-1βcontents,and CAT,GSH-Px activities in homogenate supernatant of PPs were measured by kits for observing the effects of CMP on mice lipid peroxida-tion and intestinal mucosal inflammatory induced by 5-Fu. Colon tissues were used for hematoxylin and eosin ( HE ) staining for the determination of the effect of CMP on mice colon histopathology, immunohistochem-istry for the protein levels of NF-κB and p-p38 . Results CMP significantly extended colon lengths,accelerate the intestinal propelling rates, reduced colonic mucosa epithelium goblet cell loss, inflammatory cells infiltra-tion,and crypt depth shallow induced by 5-Fu. CMP obviously reduced ROS and IL-1β contents, and pre-vented reductions in homogenate supernatant of PPs GSH content, CATand GSH-Px activities by 5-Fu ad-ministration,and also reduced the expression of NF-κB and p-p38 in colon tissues. However, CMP alone had no effect on the colon of normal mice. Conclusion The current study demonstrates that CMP may have sig-nificant protective effects against 5-Fu-induced intesti-nal mucositis. Its mechanism may be related to enhan-cing the antioxidant activity,anti-inflammatory and an-ti-apoptotic effects.关键词
羧甲基茯苓多糖/抗氧化/氟尿嘧啶/肠黏膜炎/肠推进/肠道保护Key words
carboxymethylpachyman/antioxidant/flu-orouracil/intestinal mucosal inflammatory/intestine propulsion/intestinal protection分类
医药卫生引用本文复制引用
王灿红,何晓山,张丽静,霍小位,刘冬羽,韩嘉媛,李立勇,曹丽..羧甲基茯苓多糖对氟尿嘧啶致肠炎小鼠的保护作用[J].中国药理学通报,2016,32(4):484-488,489,6.基金项目
国家科技部“重大新药创制”科技重大专项课题-早期成药性评价关键技术(No 2012ZX09501001-004) （No 2012ZX09501001-004）
国家科技部“重大新药创制”科技重大专项-综合性新药研究开发技术大平台-创新药物研究开发技术平台建设( No 2012ZX09301-002-001-026) （ No 2012ZX09301-002-001-026）
