中国药理学通报2016，Vol.32Issue(4)：489-493,5.DOI:10.3969/j.issn.1001-1978.2016.03.010
黄芪多糖抑制NF-κB/MAPK信号通路和改善哮喘大鼠气道炎症的作用
Astragalus polysaccharide regulates NF-κB/MAPK signaling pathway and attenuates airway inflammation in OVA-induced asthmatic rats
摘要
Abstract
Aim To detect the possible ameliorative effects of APS on the airway inflammation and whether the effects are associated with inhibiting the NF-κB/MAPK signaling pathway in ovalbumin( OVA)-induced asthmatic rats. Methods Asthma was induced by OVA sensitization and challenge. The asthmatic rats in the APS group were treated with APS. Pulmonary in-flammation was assessed with hematoxylin and eosin staining and inflammatory cell counting in BALF. Ul-trastructural changes of type I pneumocyte were ob-served by electron microscopy. Levels of NF-κB p65, p-NF-κB p65, p-IκBα, ERK1/2, p-ERK1/2, JNK, p-JNK, p38 MAPK, p-p38 MAPK, IL-1β, IL-4, IL-5 , IL-6 and IL-13 were measured using ELISA to as-sess the activity of NF-κB/ MAPK signaling pathway. Results Compared to the normal group, OVA in-duced significantly pulmonary inflammation and ultra-structural changes of type I pneumocyte in the asthma group. Besides, OVA increased the activity of the NF-κB/MAPK signaling pathway and promoted the produc-tion of inflammatory cytokines IL-1β, IL-4 , IL-5 , IL-6 and IL-13 in the asthma group. If compared to the asthma group, APS markedly attenuated the pulmonary inflammation and type I pneumocyte damage, as well as inhibited the activity of the NF-κB/MAPK signaling pathway and decreased the production of inflammatory cytokines in the APS group. Conclusion The amelio-rative effects of APS on airway inflammation might be associated with the inhibition of the activity of the NF-κB/MAPK signaling pathway.关键词
黄芪多糖/哮喘/炎症/NF-κB/MAPK信号通路/疗效/机制Key words
astragalus polysaccharide/asthma/in-flammation/NF-κB/MAPK signaling pathway/effect/mechanism分类
医药卫生引用本文复制引用
王金磊,李承德,孙宏伟,毛淑梅,张晓俊,曲梅花..黄芪多糖抑制NF-κB/MAPK信号通路和改善哮喘大鼠气道炎症的作用[J].中国药理学通报,2016,32(4):489-493,5.基金项目
山东省自然科学基金资助项目( No ZR2009CL045, ZR2011HL066, ZR2013CM033, ZR2015CL029) （ No ZR2009CL045, ZR2011HL066, ZR2013CM033, ZR2015CL029）
山东省教育厅科技计划项目(No J15LL54,J14LL55) （No J15LL54,J14LL55）
山东省中医药管理局项目(No 2013-242,2015-237) （No 2013-242,2015-237）
