中国药理学通报2016，Vol.32Issue(4)：494-497,498,5.DOI:10.3969/j.issn.1001-1978.2016.04.011
人参皂苷Re对H9 c2心肌细胞CYP450酶的影响
Effect of ginsenoside Re on cytochrome P450 in H9 c2 cells
摘要
Abstract
Aim To observe the cytochrome 450 effect of ginsenoside Re on H9c2 cells, in order to clarify the molecular mechanism of ginsenoside Re. Methods H 9 c 2 cells were separately treated with ginsenoside Re for 1, 5, 10, 50, 100 μmol·L-1 or 6, 24, 36, 48, 60 h. CYP2C11, 2J3, 4A1, 4A3, 4F4 and ANP mR-NA expressions were analyzed by Real time PCR, and CYP4 A1 , 2 J3 protein expressions were detected by Western blot. Results Compared with control group, ginsenoside Re could effectively upregulate CYP2 C11 , CYP2 J3 , ANP mRNA expression to 1. 6 , 1. 8 , 3. 2 fold, and downregulate CYP4A1, CYP4A3, CYP4F4 mRNA expression to 0. 4, 0. 15, 0. 3 fold. Ginsen-oside Re could decrease CYP4 A1 protein expression in a concentration-dependent manner, while ginsenoside Re could increase CYP2 J3 protein expression in a con-centration-dependent manner. Conclusion Ginsen-oside could regulate CYP450 enzyme and change ANP gene expression, which might be the molecular mecha-nism of ginsenoside Re.关键词
人参皂苷Re/心肌细胞/利钠肽/细胞色素P450酶/CYP2J3/CYP4A1Key words
ginsenoside Re/cardiac myocyte/atrial natriuretic peptide/cytochrome 450/cytochrome 2 J3/cytochrome 4 A1分类
医药卫生引用本文复制引用
马增春,高月,肖勇,赵佳伟,王宇光,谭洪玲,梁乾德,汤响林,肖成荣,杨凌..人参皂苷Re对H9 c2心肌细胞CYP450酶的影响[J].中国药理学通报,2016,32(4):494-497,498,5.基金项目
国家重点基础研究发展计划(No 2012CB518402) （No 2012CB518402）
国家自然科学基金资助项目( No 81274127) （ No 81274127）
国家科技重大专项(No 2014ZX09J14104-01A) （No 2014ZX09J14104-01A）
