中国药理学通报2016，Vol.32Issue(4)：542-547,6.DOI:10.3969/j.issn.1001-1978.2016.04.020
基于细胞自噬的荭草苷抗心肌缺血/再灌注损伤的机制研究
Cardioprotection of orientin against myocardial ischemia reperfusion injury through induction of autophagy
摘要
Abstract
Aim To investigate the role of autophagy in the cardioprotection by orientin and the relative molec-ular mechanisms in cell apoptosis and autophay. Meth-ods The isolated ischemia reperfusion ( I/R ) heart model was built firstly. The Experiments were divided into seven groups:control group, I/R group, different concentrations of orientin-treated group ( 1. 0 mg · kg-1 ,2. 0 mg· kg-1 ,4. 0 mg · kg-1 ) , autophagy in-hibitor group and resveratrol group. Hemodynamic in-dex were recorded by PowerLab, the activities of myo-cardial enzymes were detected through Biochemical an-alyzer, the ultrastructure changes and autophagosomes in myocardial cells were detected by electron microsco-py, the apoptosis was detected by TUNEL, and LC3 and Beclin1 protein levels of left ventricle were meas-ured by Western blot. Results Orientin at middle and high concentrations(2 and 4 mg·kg-1 ) induced auto-phagy shown by increased the number of autophago-somes, LC3-Ⅱ/LC3-Ⅰ ratio and Beclin 1 expression after ischemia-reperfusion. The induction of autophagy by orientin was correlated with enhanced cardiac func-tion and decreased apoptosis. But wortmannin, a kind of autophagy inhibitor, significantly attenuated the ori-entin-induced autophagy and increased apoptosis. Con-clusion The cardioprotection of orientin against myo-cardial ischemia reperfusion injury may be mediated through the inhibition of apoptosis and induction of au-tophagy.关键词
荭草苷/心肌缺血/再灌注损伤/自噬/凋亡/LC3/Be-clin 1Key words
orientin/myocardial ischemia reperfusion injury/autophagy/apoptosis/LC3/Beclin 1分类
医药卫生引用本文复制引用
刘立亚,王慧晔,黄秀兰..基于细胞自噬的荭草苷抗心肌缺血/再灌注损伤的机制研究[J].中国药理学通报,2016,32(4):542-547,6.基金项目
国家自然科学基金面上项目(No 81173656) （No 81173656）
