中国药理学通报2016，Vol.32Issue(4)：559-563,564,6.DOI:10.3969/j.issn.1001-1978.2016.04.023
人参总皂苷促进血管新生改善急性心肌梗死大鼠心功能
Total ginsenosides improve cardiac function via promoting angiogenesis in a rat model of acute myocardial infarction
摘要
Abstract
Aim To investigate the effects of total gin-senosides ( TG) on microvascular regeneration and car-diac function in rat after acute myocardial infarction ( AMI ) . Methods The acute myocardial infarction model was created with left coronary artery ligated in male Sprague Dawley rats. The model rats were ran-domly divided into sham, model, TG low and high dose groups. TG groups were injected into abdominal cavity with TG(20 mg·kg-1·d-1, 40 mg·kg-1· d-1 ) . Sham and model groups were injected with e-qual-volume normal saline. On the 35th day post-opera-tion, heart function was examined by color doppler ul-trasoundination. HE, Masson and immunohistochemis-trical staining were used to observe the histopathologi-cal changes of myocardium and micro vessel density. The level of vascular endothelial growth factor( VEGF) and basic fibroblast growth factor( bFGF) mRNA were detected by real-time PCR. Results Compared with the model group, high and low dose TG obviously de-creased the left ventricular end diastolic dimension, the left ventricular end-systolic dimension, the left ventric-ular end-diastolic volume and the left ventricular end-systolic volume(P<0. 05 and P<0. 01), and signifi-cantly increased the ejection fraction and the fractional shortening ( P <0. 01 ) . The histopathological changes of myocardium on myocardial infarction and fibrosis were dramatically reduced by TG. But ventricular wall was thicker. Two dose TG remarkably increased the expressions of VEGF and bFGF mRNA and micro ves-sel density of compositive CD31 + cells in ischemic myocardial tissue and around of infarct area(P<0. 01, P <0 . 0 5 ) . Conclusions TG could improve the car-diac function of acute myocardial infarction rat. The mechanism may be related to the upregulation of VEGF and bFGF gene expression, the promotion angiogene-sis, then the improvement of blood supply in myocardi-al infarction area.关键词
人参总皂苷/急性心肌梗死/心功能/血管内皮生长因子/碱性成纤维细胞生长因子/血管新生/心室重构Key words
total ginsenosides/acute myocardial in-farction/cardiac function/vascular endothelial growth factor/basic fibroblast growth factor/angiogenesis/myocardial remodeling分类
医药卫生引用本文复制引用
魏英,余丽梅,王钰莹,姚观平,潘丽..人参总皂苷促进血管新生改善急性心肌梗死大鼠心功能[J].中国药理学通报,2016,32(4):559-563,564,6.基金项目
贵州省科技厅攻关项目黔科合S字[2007]1029 （）
遵义医学院博士后基金遵医(2006)034 （2006）
