中国药理学通报2016，Vol.32Issue(4)：581-584,585,5.DOI:10.3969/j.issn.1001-1978.2016.04.027
用于肿瘤细胞三维培养的微流控芯片构建及培养条件研究
Microfluidic chip for tumor cell 3 D culturing establishment and its cultural conditions
摘要
Abstract
Aims To design and fabricate the 3 D cell cultural microfluidic chip for tumor cell culturing, with which to research the compatible conditions for gelatin forming and dissolving with calcium alginate as the scaffolds. To culture SMMC-7721 cells in the chip and to detect the surviving rate. Methods The microfluid-ic chip was fabricated with the software Corel Draw, the technology of soft lithography, molding, and plas-ma bonding. The applicability was tested and cells were cultured on it, on which the cell status was ob-served, their surviving rate was calculated with the help of the software IPP. Results The chip we fabri-cate was calculated is suitable for cell 3D culturing, the tumor cells showed a favorable proliferation ability in 72 h on chip, the surviving rate was ( 96. 1 ± 4. 5)%. The cells were solid and TCS appeared. Con-clusion The microfluidic chip manufactured appeared for tumor cell 3D culture, is suitable for the growth of SMMC-7721 and the cells are indubitable. They show some different status in proliferative and agminated compared with traditional 2D cell culture. With the chip and the condition found, there will be a better way to study the characteristics of tumor cells and is beneficial to the screen of anti-tumor drugs.关键词
微流控芯片/肿瘤细胞/肿瘤微环境/细胞三维培养/海藻酸钙凝胶/培养条件Key words
microfluidic chip/tumor cell/tumor mi-croenvironment/three-dimensional cell culture/sodium alginate/culture condition分类
医药卫生引用本文复制引用
徐为峰,王帅,孟宪生,包永睿..用于肿瘤细胞三维培养的微流控芯片构建及培养条件研究[J].中国药理学通报,2016,32(4):581-584,585,5.基金项目
国家“十二五”重大新药创制专项( No 2013ZX09507005-005) （ No 2013ZX09507005-005）
