中国药业2016，Vol.25Issue(1)：49-52,4.
水飞蓟宾脂质体制备方法研究
Studies on the Preparation Methods of Silybin Liposomes
何文 1胡翠萍 2郭咸希1
作者信息
- 1. 武汉大学人民医院，湖北 武汉 430060
- 2. 武汉大学药学院，湖北 武汉 430071
- 折叠
摘要
Abstract
Objective To screen the best method to prepare silybin liposomes. Methods Silybin liposomes were respectively prepared by film dispersion method, multiple emulsion method, and reverse evaporation method. The morphological characteristic of the liposomes was observed by optical microscope. The filter membrane method was employed to isolate free silybin, and HPLC was used to determine the drug content and the entrapment efficiency of silybin liposomes. The entrapment efficiency and particle size distribution as the indices, the best prepareration method was chosen. Results Before high-speed shearing, the liposomes respectively prepared by the three meth-ods are spheroidal without no difference. The entrapment efficiency of the liposomes respectively prepared by film dispersion method, multiple emulsion method and reverse evaporation method, was respectively ( 88. 67 ± 16 )%, ( 68. 88 ± 1. 5 ) % and ( 59. 96 ± 1. 2 )%;while the encapsulation efficiency was respectively ( 72. 32 ± 7. 6 )%, ( 31. 68 ± 3. 1 )% and ( 37. 66 ± 1. 4 )% after high-speed shear-ing. The mean diameter of silybin liposomes were ( 134. 8 ± 2. 5 ) nm, ( 138. 5 ± 2. 5 ) nm and ( 165. 0 ± 1. 1 ) nm after high-speed shear-ing. The polydispersity indeice were 0. 235, 0. 182 and 0. 233, respectively. Conclusion The encapsulation efficiency of silybin liposomes prepared by the film dispersion method is the highest, and the effect of decreasing size on the entrapment efficiency is not evident, which can provide experimental foundation for the further study of the liver targeting preparation.关键词
水飞蓟宾/脂质体/包封率/多分散性指数Key words
silybin/liposomes/encapsulation efficiency/polydispersity index分类
医药卫生引用本文复制引用
何文,胡翠萍,郭咸希..水飞蓟宾脂质体制备方法研究[J].中国药业,2016,25(1):49-52,4.
