中国药业2016，Vol.25Issue(1)：58-60,3.
高效液相色谱法同时测定土贝母中土贝母苷甲、乙和丙的含量
Determination of Tubeimoside Ⅰ, Tubeimoside Ⅱand Tubeimoside Ⅲ in Bolbostemma paniculatum (Maxim.) Franquet by HPLC
摘要
Abstract
Objective To develop an HPLC method for content determination of tubeimoside Ⅰ, t-ubeimoside Ⅱand tubeimoside Ⅲ in Bolbostemma paniculatum ( Maxim. ) Franquet. Methods The determination was carried out on a C18 column ( 250 mm × 4. 6 mm, 5 μm ) eluted with acetonitrile and water. The flow rate was 1. 0 mL/min, and the detected wavelength was set 214 nm. Results The peak ar-eas and the sample quantity of the three components had good linear relationship in the range of 1. 28~12. 8 μg for tubeimoside Ⅰ, 0. 241~2. 41 μg for tubeimoside Ⅱ and 0. 515~5. 15 μg for tubeimoside Ⅲ. The average recoveries were 99. 10%, 98. 95% and 98. 87%, respectively. Conclusion The method was proved to be simple, accurate and provided reference for comprehensive quality con-trol of Bolbostemma paniculatum ( Maxim. ) Franquet.关键词
土贝母/高效液相色谱法/土贝母苷甲/土贝母苷乙/土贝母苷丙Key words
Bolbostemma paniculatum ( Maxim. ) Franquet/HPLC/tubeimoside Ⅰ/tubeimoside Ⅱ/tubeimoside Ⅲ分类
医药卫生引用本文复制引用
黄瑾,张杰,顾正兵..高效液相色谱法同时测定土贝母中土贝母苷甲、乙和丙的含量[J].中国药业,2016,25(1):58-60,3.基金项目
上海市浦东新区卫生局面上项目，项目编号PW2013A－12。 （）
