中国药业2016，Vol.25Issue(1)：61-62,63,3.
高效液相色谱法分离与测定盐酸多塞平顺反式异构体的含量
Separation and Determination of ZE-Doxepin Hydrochloride by HPLC
韩春晖 1张春泓 1栾爽1
作者信息
- 1. 辽宁省大连市药品检验所，辽宁 大连 116021
- 折叠
摘要
Abstract
Objective To establish a method for the separation and determination of Doxepin Hydrochloride in Doxepin Hydrochloride Cream by HPLC. Methods Chromatography was performed on a Kromasil 100A C18 ( 150 mm × 4. 6 mm, 5 μm ) column. The mobile phase was consisted of 0. 1% triethylamine in 0. 2 mol/L sodium dihydrogen phosohate solution-methanol ( 65:35 ) . The flow rate was 1. 0 mL/min. The detection wavelength was 254 nm. The column temperature was 50 ℃. Results The linear response range was 7. 90~12. 66 μg/mL for Doxepin Hydrochloride ( r=0. 999 9 ) . The recovery of Doxepin Hydrochloride was 98. 88% ( RSD=1. 32%,n=9 ) . Conclusion The method can separate ZE-doxepin hydrochloride, which was suitable for the determination of Doxepin Hydrochloride in doxepin hydrochloride cream.关键词
高效液相色谱法/盐酸多塞平/含量测定/异构体Key words
HPLC/doxepin hydrochloride/determination/isomer分类
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韩春晖,张春泓,栾爽..高效液相色谱法分离与测定盐酸多塞平顺反式异构体的含量[J].中国药业,2016,25(1):61-62,63,3.
